Matches in SemOpenAlex for { <https://semopenalex.org/work/W2017008847> ?p ?o ?g. }
Showing items 1 to 93 of
93
with 100 items per page.
- W2017008847 abstract "Sequencing the human genome is a major undertaking considering the large number of nucleotides present in the genome and the slow methods currently available to perform the task. The authors have recently reported on a scheme to sequence DNA rapidly using a non-gel based technique. The concept is based upon the incorporation of fluorescently labeled nucleotides into a strand of DNA, isolation and manipulation of a labeled DNA fragment and the detection of single nucleotides using ultra-sensitive laser-induced fluorescence detection following their cleavage from the fragment. Detection of individual fluorophores in the liquid phase was accomplished with time-gated detection following pulsed-laser excitation. The photon bursts from individual rhodamine 6G (R6G) molecules travelling through a laser beam have been observed, as have bursts from single fluorescently modified nucleotides. Using two different biotinylated nucleotides as a model system for fluorescently labeled nucleotides, the authors have observed synthesis of the complementary copy of M13 bacteriophage. Work with fluorescently labeled nucleotides is underway. Individual molecules of DNA attached to a microbead have been observed and manipulated with an epifluorescence microscope." @default.
- W2017008847 created "2016-06-24" @default.
- W2017008847 creator A5001041725 @default.
- W2017008847 creator A5003281170 @default.
- W2017008847 creator A5010208544 @default.
- W2017008847 creator A5012183092 @default.
- W2017008847 creator A5015792976 @default.
- W2017008847 creator A5017994736 @default.
- W2017008847 creator A5024891495 @default.
- W2017008847 creator A5038208939 @default.
- W2017008847 creator A5040654578 @default.
- W2017008847 creator A5065259104 @default.
- W2017008847 creator A5081430818 @default.
- W2017008847 creator A5089735473 @default.
- W2017008847 date "1991-07-01" @default.
- W2017008847 modified "2023-09-27" @default.
- W2017008847 title "<title>Rapid sequencing of DNA based on single-molecule detection</title>" @default.
- W2017008847 doi "https://doi.org/10.1117/12.44241" @default.
- W2017008847 hasPublicationYear "1991" @default.
- W2017008847 type Work @default.
- W2017008847 sameAs 2017008847 @default.
- W2017008847 citedByCount "5" @default.
- W2017008847 crossrefType "proceedings-article" @default.
- W2017008847 hasAuthorship W2017008847A5001041725 @default.
- W2017008847 hasAuthorship W2017008847A5003281170 @default.
- W2017008847 hasAuthorship W2017008847A5010208544 @default.
- W2017008847 hasAuthorship W2017008847A5012183092 @default.
- W2017008847 hasAuthorship W2017008847A5015792976 @default.
- W2017008847 hasAuthorship W2017008847A5017994736 @default.
- W2017008847 hasAuthorship W2017008847A5024891495 @default.
- W2017008847 hasAuthorship W2017008847A5038208939 @default.
- W2017008847 hasAuthorship W2017008847A5040654578 @default.
- W2017008847 hasAuthorship W2017008847A5065259104 @default.
- W2017008847 hasAuthorship W2017008847A5081430818 @default.
- W2017008847 hasAuthorship W2017008847A5089735473 @default.
- W2017008847 hasBestOaLocation W20170088473 @default.
- W2017008847 hasConcept C104317684 @default.
- W2017008847 hasConcept C121332964 @default.
- W2017008847 hasConcept C12554922 @default.
- W2017008847 hasConcept C13575015 @default.
- W2017008847 hasConcept C153911025 @default.
- W2017008847 hasConcept C178790620 @default.
- W2017008847 hasConcept C185592680 @default.
- W2017008847 hasConcept C2776363448 @default.
- W2017008847 hasConcept C2777829344 @default.
- W2017008847 hasConcept C3017666073 @default.
- W2017008847 hasConcept C32909587 @default.
- W2017008847 hasConcept C512185932 @default.
- W2017008847 hasConcept C51679486 @default.
- W2017008847 hasConcept C54887055 @default.
- W2017008847 hasConcept C552990157 @default.
- W2017008847 hasConcept C55493867 @default.
- W2017008847 hasConcept C62520636 @default.
- W2017008847 hasConcept C86803240 @default.
- W2017008847 hasConcept C91881484 @default.
- W2017008847 hasConceptScore W2017008847C104317684 @default.
- W2017008847 hasConceptScore W2017008847C121332964 @default.
- W2017008847 hasConceptScore W2017008847C12554922 @default.
- W2017008847 hasConceptScore W2017008847C13575015 @default.
- W2017008847 hasConceptScore W2017008847C153911025 @default.
- W2017008847 hasConceptScore W2017008847C178790620 @default.
- W2017008847 hasConceptScore W2017008847C185592680 @default.
- W2017008847 hasConceptScore W2017008847C2776363448 @default.
- W2017008847 hasConceptScore W2017008847C2777829344 @default.
- W2017008847 hasConceptScore W2017008847C3017666073 @default.
- W2017008847 hasConceptScore W2017008847C32909587 @default.
- W2017008847 hasConceptScore W2017008847C512185932 @default.
- W2017008847 hasConceptScore W2017008847C51679486 @default.
- W2017008847 hasConceptScore W2017008847C54887055 @default.
- W2017008847 hasConceptScore W2017008847C552990157 @default.
- W2017008847 hasConceptScore W2017008847C55493867 @default.
- W2017008847 hasConceptScore W2017008847C62520636 @default.
- W2017008847 hasConceptScore W2017008847C86803240 @default.
- W2017008847 hasConceptScore W2017008847C91881484 @default.
- W2017008847 hasLocation W20170088471 @default.
- W2017008847 hasLocation W20170088472 @default.
- W2017008847 hasLocation W20170088473 @default.
- W2017008847 hasOpenAccess W2017008847 @default.
- W2017008847 hasPrimaryLocation W20170088471 @default.
- W2017008847 hasRelatedWork W2016218069 @default.
- W2017008847 hasRelatedWork W2017008847 @default.
- W2017008847 hasRelatedWork W2020181395 @default.
- W2017008847 hasRelatedWork W2068505713 @default.
- W2017008847 hasRelatedWork W2087418749 @default.
- W2017008847 hasRelatedWork W2121531343 @default.
- W2017008847 hasRelatedWork W2408933369 @default.
- W2017008847 hasRelatedWork W2418987332 @default.
- W2017008847 hasRelatedWork W2769869113 @default.
- W2017008847 hasRelatedWork W4243210338 @default.
- W2017008847 isParatext "false" @default.
- W2017008847 isRetracted "false" @default.
- W2017008847 magId "2017008847" @default.
- W2017008847 workType "article" @default.