FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2017013414> ?p ?o ?g. }

• W2017013414 endingPage "830" @default.
• W2017013414 startingPage "819" @default.
• W2017013414 abstract "The objective was to determine the optimal concentration of heparin for sperm capacitation, as well as the optimal sperm concentration for in vitro fertilization using flow cytometrically-sorted sperm from individual bulls. A total of 5327 bovine oocytes and sperm from four bulls were examined. Oocytes from slaughterhouse ovaries were matured in TCM199 for 22–24 h. Flow cytometrically-sorted sperm as well as unsorted control sperm from the same bulls were cryopreserved. For sperm from each of the four bulls, oocytes were inseminated in a three-by-three factorial design plus one control group (three heparin concentrations: 0, 2, and 10 μg/ml and three sperm concentrations: 0.5×106, 1.5×106, and 4.5×106 ml−1; 10 μg/ml of heparin and 1.5×106 ml−1 of sperm were used for the unsorted control). Presumptive zygotes were cultured in chemically defined media, CDM-1 and CDM-2 for 52–54 h and 96 h, respectively. Samples of about 10 oocytes from each of the 10 treatment groups per replicate were fixed at 18–20 h after insemination to determine sperm pronuclei formation and polyspermy. Increased polyspermy resulted as heparin and sperm concentrations increased (P<0.05). A higher rate of polyspermy was found in oocytes inseminated with unsorted control sperm compared with sorted sperm (P<0.05). Sperm of one of four bulls tested required no heparin and lower concentration (0.5×106 ml−1) to obtain optimal cleavage and blastocyst rates while optimal parameters for another bull were higher heparin (10 μg/ml) and sperm concentrations (4.5×106 ml−1). Optimal parameters for the other two were intermediate levels of heparin and sperm. Sperm appeared to be partially capacitated during the flow cytometric-sorting process used for sex pre-determination. When heparin and sperm concentrations were optimized for individual bulls, blastocyst production per oocyte was similar for sorted and unsorted sperm for three of the four bulls studied." @default.
• W2017013414 created "2016-06-24" @default.
• W2017013414 creator A5044191015 @default.
• W2017013414 creator A5053755237 @default.
• W2017013414 date "2004-09-01" @default.
• W2017013414 modified "2023-10-18" @default.
• W2017013414 title "Effects of heparin and sperm concentration on cleavage and blastocyst development rates of bovine oocytes inseminated with flow cytometrically-sorted sperm" @default.
• W2017013414 cites W1997209743 @default.
• W2017013414 cites W2006668497 @default.
• W2017013414 cites W2026663081 @default.
• W2017013414 cites W2040080912 @default.
• W2017013414 cites W2051038137 @default.
• W2017013414 cites W2072526865 @default.
• W2017013414 cites W2079812680 @default.
• W2017013414 cites W2081349558 @default.
• W2017013414 cites W2083211009 @default.
• W2017013414 cites W2091415696 @default.
• W2017013414 cites W2093467583 @default.
• W2017013414 cites W2106720945 @default.
• W2017013414 cites W2152908680 @default.
• W2017013414 cites W2153265419 @default.
• W2017013414 cites W2173190486 @default.
• W2017013414 doi "https://doi.org/10.1016/j.theriogenology.2003.12.029" @default.
• W2017013414 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/15251233" @default.
• W2017013414 hasPublicationYear "2004" @default.
• W2017013414 type Work @default.
• W2017013414 sameAs 2017013414 @default.
• W2017013414 citedByCount "68" @default.
• W2017013414 countsByYear W20170134142012 @default.
• W2017013414 countsByYear W20170134142013 @default.
• W2017013414 countsByYear W20170134142014 @default.
• W2017013414 countsByYear W20170134142015 @default.
• W2017013414 countsByYear W20170134142016 @default.
• W2017013414 countsByYear W20170134142017 @default.
• W2017013414 countsByYear W20170134142018 @default.
• W2017013414 countsByYear W20170134142020 @default.
• W2017013414 countsByYear W20170134142021 @default.
• W2017013414 countsByYear W20170134142022 @default.
• W2017013414 countsByYear W20170134142023 @default.
• W2017013414 crossrefType "journal-article" @default.
• W2017013414 hasAuthorship W2017013414A5044191015 @default.
• W2017013414 hasAuthorship W2017013414A5053755237 @default.
• W2017013414 hasConcept C105702510 @default.
• W2017013414 hasConcept C110758877 @default.
• W2017013414 hasConcept C127315564 @default.
• W2017013414 hasConcept C159879943 @default.
• W2017013414 hasConcept C16685009 @default.
• W2017013414 hasConcept C179933525 @default.
• W2017013414 hasConcept C185592680 @default.
• W2017013414 hasConcept C196843134 @default.
• W2017013414 hasConcept C2777005246 @default.
• W2017013414 hasConcept C2778093475 @default.
• W2017013414 hasConcept C2778177303 @default.
• W2017013414 hasConcept C2780301668 @default.
• W2017013414 hasConcept C2781087480 @default.
• W2017013414 hasConcept C28748434 @default.
• W2017013414 hasConcept C2994278967 @default.
• W2017013414 hasConcept C512716672 @default.
• W2017013414 hasConcept C54355233 @default.
• W2017013414 hasConcept C71924100 @default.
• W2017013414 hasConcept C86803240 @default.
• W2017013414 hasConcept C87073359 @default.
• W2017013414 hasConcept C88972607 @default.
• W2017013414 hasConcept C90642696 @default.
• W2017013414 hasConceptScore W2017013414C105702510 @default.
• W2017013414 hasConceptScore W2017013414C110758877 @default.
• W2017013414 hasConceptScore W2017013414C127315564 @default.
• W2017013414 hasConceptScore W2017013414C159879943 @default.
• W2017013414 hasConceptScore W2017013414C16685009 @default.
• W2017013414 hasConceptScore W2017013414C179933525 @default.
• W2017013414 hasConceptScore W2017013414C185592680 @default.
• W2017013414 hasConceptScore W2017013414C196843134 @default.
• W2017013414 hasConceptScore W2017013414C2777005246 @default.
• W2017013414 hasConceptScore W2017013414C2778093475 @default.
• W2017013414 hasConceptScore W2017013414C2778177303 @default.
• W2017013414 hasConceptScore W2017013414C2780301668 @default.
• W2017013414 hasConceptScore W2017013414C2781087480 @default.
• W2017013414 hasConceptScore W2017013414C28748434 @default.
• W2017013414 hasConceptScore W2017013414C2994278967 @default.
• W2017013414 hasConceptScore W2017013414C512716672 @default.
• W2017013414 hasConceptScore W2017013414C54355233 @default.
• W2017013414 hasConceptScore W2017013414C71924100 @default.
• W2017013414 hasConceptScore W2017013414C86803240 @default.
• W2017013414 hasConceptScore W2017013414C87073359 @default.
• W2017013414 hasConceptScore W2017013414C88972607 @default.
• W2017013414 hasConceptScore W2017013414C90642696 @default.
• W2017013414 hasIssue "5" @default.
• W2017013414 hasLocation W20170134141 @default.
• W2017013414 hasLocation W20170134142 @default.
• W2017013414 hasOpenAccess W2017013414 @default.
• W2017013414 hasPrimaryLocation W20170134141 @default.
• W2017013414 hasRelatedWork W1990334492 @default.
• W2017013414 hasRelatedWork W2017013414 @default.
• W2017013414 hasRelatedWork W2030293483 @default.
• W2017013414 hasRelatedWork W2054088622 @default.
• W2017013414 hasRelatedWork W2090437060 @default.
• W2017013414 hasRelatedWork W2135098535 @default.
• W2017013414 hasRelatedWork W2262920417 @default.

• next