Matches in SemOpenAlex for { <https://semopenalex.org/work/W2017014411> ?p ?o ?g. }
Showing items 1 to 95 of
95
with 100 items per page.
- W2017014411 endingPage "151" @default.
- W2017014411 startingPage "147" @default.
- W2017014411 abstract "13C NMR studies of 13C-labelled ligands bound to dihydrofolate reductase provide (DHFR) a powerful means of detecting and characterizing multiple bound conformations. Such studies of complexes of Escherichia coli DHFR with [4,7,8a,9-13C]- and [2,4a,6-13C]methotrexate (MTX) and [4,6,8a-13C]- and [2,4a,7,9-13C]folic acid confirm that in the binary complexes, MTX binds in two conformational forms and folate binds as a single conformation. Earlier studies on the corresponding complexes with Lactobacillus casei DHFR indicated that, in this case, MTX binds as a single conformation whereas folate binds in multiple conformational forms (both in its binary complex and ternary complex with NADP+); two of the bound conformational states for the folate complexes are very different from each other in that there is a 180 degrees difference in their pteridine ring orientation. In contrast, the two different conformational states observed for MTX bound to E. coli DHFR do not show such a major difference in ring orientation and bind with N1 protonated in both forms. The major difference appears to involve the manner in which the 4-NH2 group of MTX binds to the enzyme (although the same protein residues are probably involved in both interactions). Addition of either NADP+ or NADPH to the E. coli DHFR-MTX complex results in a single set of 13C signals for bound methotrexate consistent with only one conformational form in the ternary complexes." @default.
- W2017014411 created "2016-06-24" @default.
- W2017014411 creator A5026595457 @default.
- W2017014411 creator A5032365073 @default.
- W2017014411 creator A5048308517 @default.
- W2017014411 date "1992-11-09" @default.
- W2017014411 modified "2023-09-26" @default.
- W2017014411 title "<sup>13</sup>C NMR studies of complexes of<i>Escherichia coli</i>dihydrofolate reductase formed with methotrexate and with folic acid" @default.
- W2017014411 cites W1979482400 @default.
- W2017014411 cites W1980326728 @default.
- W2017014411 cites W1994643869 @default.
- W2017014411 cites W1994911131 @default.
- W2017014411 cites W2002102692 @default.
- W2017014411 cites W2012758072 @default.
- W2017014411 cites W2051882793 @default.
- W2017014411 cites W2055633870 @default.
- W2017014411 cites W2063716762 @default.
- W2017014411 cites W2065124616 @default.
- W2017014411 cites W2090784557 @default.
- W2017014411 cites W2093883240 @default.
- W2017014411 cites W2104099472 @default.
- W2017014411 cites W2116493634 @default.
- W2017014411 cites W2161117347 @default.
- W2017014411 cites W2206965731 @default.
- W2017014411 cites W2399865230 @default.
- W2017014411 doi "https://doi.org/10.1016/0014-5793(92)80923-5" @default.
- W2017014411 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/1426244" @default.
- W2017014411 hasPublicationYear "1992" @default.
- W2017014411 type Work @default.
- W2017014411 sameAs 2017014411 @default.
- W2017014411 citedByCount "14" @default.
- W2017014411 countsByYear W20170144112020 @default.
- W2017014411 crossrefType "journal-article" @default.
- W2017014411 hasAuthorship W2017014411A5026595457 @default.
- W2017014411 hasAuthorship W2017014411A5032365073 @default.
- W2017014411 hasAuthorship W2017014411A5048308517 @default.
- W2017014411 hasBestOaLocation W20170144111 @default.
- W2017014411 hasConcept C100544194 @default.
- W2017014411 hasConcept C104317684 @default.
- W2017014411 hasConcept C145148216 @default.
- W2017014411 hasConcept C178790620 @default.
- W2017014411 hasConcept C181199279 @default.
- W2017014411 hasConcept C185592680 @default.
- W2017014411 hasConcept C2776275976 @default.
- W2017014411 hasConcept C2776662696 @default.
- W2017014411 hasConcept C2777339483 @default.
- W2017014411 hasConcept C2778397455 @default.
- W2017014411 hasConcept C2781320022 @default.
- W2017014411 hasConcept C2781382166 @default.
- W2017014411 hasConcept C29730261 @default.
- W2017014411 hasConcept C30095370 @default.
- W2017014411 hasConcept C547475151 @default.
- W2017014411 hasConcept C55493867 @default.
- W2017014411 hasConcept C71240020 @default.
- W2017014411 hasConcept C86756495 @default.
- W2017014411 hasConceptScore W2017014411C100544194 @default.
- W2017014411 hasConceptScore W2017014411C104317684 @default.
- W2017014411 hasConceptScore W2017014411C145148216 @default.
- W2017014411 hasConceptScore W2017014411C178790620 @default.
- W2017014411 hasConceptScore W2017014411C181199279 @default.
- W2017014411 hasConceptScore W2017014411C185592680 @default.
- W2017014411 hasConceptScore W2017014411C2776275976 @default.
- W2017014411 hasConceptScore W2017014411C2776662696 @default.
- W2017014411 hasConceptScore W2017014411C2777339483 @default.
- W2017014411 hasConceptScore W2017014411C2778397455 @default.
- W2017014411 hasConceptScore W2017014411C2781320022 @default.
- W2017014411 hasConceptScore W2017014411C2781382166 @default.
- W2017014411 hasConceptScore W2017014411C29730261 @default.
- W2017014411 hasConceptScore W2017014411C30095370 @default.
- W2017014411 hasConceptScore W2017014411C547475151 @default.
- W2017014411 hasConceptScore W2017014411C55493867 @default.
- W2017014411 hasConceptScore W2017014411C71240020 @default.
- W2017014411 hasConceptScore W2017014411C86756495 @default.
- W2017014411 hasIssue "2-3" @default.
- W2017014411 hasLocation W20170144111 @default.
- W2017014411 hasLocation W20170144112 @default.
- W2017014411 hasOpenAccess W2017014411 @default.
- W2017014411 hasPrimaryLocation W20170144111 @default.
- W2017014411 hasRelatedWork W1982669395 @default.
- W2017014411 hasRelatedWork W1984777478 @default.
- W2017014411 hasRelatedWork W2017014411 @default.
- W2017014411 hasRelatedWork W2062433492 @default.
- W2017014411 hasRelatedWork W2114699933 @default.
- W2017014411 hasRelatedWork W2121814459 @default.
- W2017014411 hasRelatedWork W2165074963 @default.
- W2017014411 hasRelatedWork W2165835219 @default.
- W2017014411 hasRelatedWork W2403112270 @default.
- W2017014411 hasRelatedWork W4250704989 @default.
- W2017014411 hasVolume "312" @default.
- W2017014411 isParatext "false" @default.
- W2017014411 isRetracted "false" @default.
- W2017014411 magId "2017014411" @default.
- W2017014411 workType "article" @default.