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• W2017015941 abstract "A method for the detection of collagenous proteins within cyanogen bromide digests of tissues has been devised. The peptides produced by digestion with cyanogen bromide were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and transferred to a nitrocellulose filter. They were stained on the filter by incubation first with antibodies to collagen and then with a second antibody covalently linked to horseradish peroxidase, 4-chloro-1-naphthol was added, and the bound enzyme was assayed. This procedure is useful for the identification and characterization of collagens of types I, III, IV, and V in tissues. In addition, we have developed a sensitive and specific competitive enzyme-linked immunosorbent assay (ELISA) which is convenient for quantifying collagens (types I, III, and IV) in tissues. In this kind of assay, soluble cyanogen bromide peptides compete with cyanogen bromide peptides adsorbed onto a solid-phase support for rabbit anti-collagen antibodies. We determined the amount of bound antibody by using goat anti-rabbit immunoglobulin G covalently conjugated to horseradish peroxidase and then provided a substrate for the enzymatic reaction. The sensitivity range of the ELISA is 0.09 μg/ml in the region of 90 to 10% binding." @default.
• W2017015941 created "2016-06-24" @default.
• W2017015941 creator A5049635748 @default.
• W2017015941 date "1985-10-01" @default.
• W2017015941 modified "2023-10-18" @default.
• W2017015941 title "Quantification and specific detection of collagenous proteins using an enzyme-linked immunosorbent assay and an immunoblotting for cyanogen bromide peptides" @default.
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• W2017015941 doi "https://doi.org/10.1016/0003-2697(85)90459-2" @default.
• W2017015941 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/4083479" @default.
• W2017015941 hasPublicationYear "1985" @default.
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