FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2017016784> ?p ?o ?g. }

• W2017016784 endingPage "e35546" @default.
• W2017016784 startingPage "e35546" @default.
• W2017016784 abstract "Laminin-332 (α3ß3γ2) (Lm332) supports the stable anchoring of basal keratinocytes to the epidermal basement membrane, while it functions as a motility factor for wound healing and cancer invasion. To understand these contrasting activities of Lm332, we investigated Lm332 matrices deposited by normal human keratinocytes and other Lm332-expressing cell lines. All types of the cells efficiently deposited Lm332 on the culture plates in specific patterns. On the contrary, laminins containing laminin ß1 and/or γ1 chains, such as Lm511 and Lm311, were not deposited on the culture plates even if secreted into culture medium. The Lm332 deposition was not inhibited by function-blocking antibodies to the α3 and α6 integrins but was inhibited by sodium selenate, suggesting that sulfated glycosaminoglycans on cell surface, e.g. heparan sulfate proteoglycans, might be involved in the process. HEK293 cells overexpressing exogenous Lm332 (Lm332-HEK) almost exclusively deposited Lm332 on the plates. The deposited Lm332 matrix showed a mesh-like network structure as analyzed by electron microscopy, suggesting that Lm332 was highly polymerized. When biological activity was analyzed, the Lm332 matrix rather suppressed the migration of keratinocytes as compared with purified Lm332, which highly promoted the cell migration. The Lm332 matrix supported adhesion of keratinocytes much more strongly and stably than purified Lm332. Integrin α3ß1 bound to the Lm332 matrix at a three times higher level than purified Lm332. Normal keratinocytes prominently showed integrin α6ß4-containing, hemidesmosome-like structures on the Lm332 matrix but not on the purified one. These results indicate that the polymerized Lm332 matrix supports stable cell adhesion by interacting with both integrin α6ß4 and α3ß1, whereas unassembled soluble Lm332 supports cell migration." @default.
• W2017016784 created "2016-06-24" @default.
• W2017016784 creator A5009882274 @default.
• W2017016784 creator A5026390892 @default.
• W2017016784 creator A5047604331 @default.
• W2017016784 creator A5057916113 @default.
• W2017016784 creator A5070427110 @default.
• W2017016784 date "2012-05-01" @default.
• W2017016784 modified "2023-09-24" @default.
• W2017016784 title "Polymerized Laminin-332 Matrix Supports Rapid and Tight Adhesion of Keratinocytes, Suppressing Cell Migration" @default.
• W2017016784 cites W1496915816 @default.
• W2017016784 cites W1839350769 @default.
• W2017016784 cites W1907627763 @default.
• W2017016784 cites W1952725897 @default.
• W2017016784 cites W1968706475 @default.
• W2017016784 cites W1972682554 @default.
• W2017016784 cites W1976022047 @default.
• W2017016784 cites W1976696904 @default.
• W2017016784 cites W1978837916 @default.
• W2017016784 cites W1982204629 @default.
• W2017016784 cites W1985491088 @default.
• W2017016784 cites W1989074212 @default.
• W2017016784 cites W1990084142 @default.
• W2017016784 cites W2002405232 @default.
• W2017016784 cites W2003280609 @default.
• W2017016784 cites W2005494249 @default.
• W2017016784 cites W2005918089 @default.
• W2017016784 cites W2008800104 @default.
• W2017016784 cites W2025361420 @default.
• W2017016784 cites W2026632078 @default.
• W2017016784 cites W2037598346 @default.
• W2017016784 cites W2041429746 @default.
• W2017016784 cites W2048845152 @default.
• W2017016784 cites W2055526203 @default.
• W2017016784 cites W2056053762 @default.
• W2017016784 cites W2063361905 @default.
• W2017016784 cites W2067693141 @default.
• W2017016784 cites W2072041699 @default.
• W2017016784 cites W2077061694 @default.
• W2017016784 cites W2077103399 @default.
• W2017016784 cites W2089747862 @default.
• W2017016784 cites W2089970204 @default.
• W2017016784 cites W2090206479 @default.
• W2017016784 cites W2090323246 @default.
• W2017016784 cites W2108295622 @default.
• W2017016784 cites W2121513486 @default.
• W2017016784 cites W2124358624 @default.
• W2017016784 cites W2133032561 @default.
• W2017016784 cites W2137652101 @default.
• W2017016784 cites W2141367881 @default.
• W2017016784 cites W2143529663 @default.
• W2017016784 cites W2148399778 @default.
• W2017016784 cites W2165524164 @default.
• W2017016784 cites W2169606620 @default.
• W2017016784 cites W2188060873 @default.
• W2017016784 cites W2803499602 @default.
• W2017016784 cites W4213141530 @default.
• W2017016784 doi "https://doi.org/10.1371/journal.pone.0035546" @default.
• W2017016784 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3341393" @default.
• W2017016784 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/22563463" @default.
• W2017016784 hasPublicationYear "2012" @default.
• W2017016784 type Work @default.
• W2017016784 sameAs 2017016784 @default.
• W2017016784 citedByCount "27" @default.
• W2017016784 countsByYear W20170167842013 @default.
• W2017016784 countsByYear W20170167842014 @default.
• W2017016784 countsByYear W20170167842015 @default.
• W2017016784 countsByYear W20170167842016 @default.
• W2017016784 countsByYear W20170167842017 @default.
• W2017016784 countsByYear W20170167842018 @default.
• W2017016784 countsByYear W20170167842019 @default.
• W2017016784 countsByYear W20170167842020 @default.
• W2017016784 countsByYear W20170167842022 @default.
• W2017016784 countsByYear W20170167842023 @default.
• W2017016784 crossrefType "journal-article" @default.
• W2017016784 hasAuthorship W2017016784A5009882274 @default.
• W2017016784 hasAuthorship W2017016784A5026390892 @default.
• W2017016784 hasAuthorship W2017016784A5047604331 @default.
• W2017016784 hasAuthorship W2017016784A5057916113 @default.
• W2017016784 hasAuthorship W2017016784A5070427110 @default.
• W2017016784 hasBestOaLocation W20170167841 @default.
• W2017016784 hasConcept C106487976 @default.
• W2017016784 hasConcept C147075424 @default.
• W2017016784 hasConcept C1491633281 @default.
• W2017016784 hasConcept C153074725 @default.
• W2017016784 hasConcept C153911025 @default.
• W2017016784 hasConcept C185592680 @default.
• W2017016784 hasConcept C189165786 @default.
• W2017016784 hasConcept C195687474 @default.
• W2017016784 hasConcept C202751555 @default.
• W2017016784 hasConcept C2776193487 @default.
• W2017016784 hasConcept C2777074287 @default.
• W2017016784 hasConcept C2778041096 @default.
• W2017016784 hasConcept C2779814568 @default.
• W2017016784 hasConcept C43617362 @default.
• W2017016784 hasConcept C54355233 @default.
• W2017016784 hasConcept C55493867 @default.
• W2017016784 hasConcept C81885089 @default.

• next