FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2017017620> ?p ?o ?g. }

• W2017017620 endingPage "106" @default.
• W2017017620 startingPage "97" @default.
• W2017017620 abstract "The mature lipase LipA and its 56aa-truncated chaperone ΔLipBhis (with 6×his-tag) from Ralstonia sp. M1 were over-expressed in Escherichia coli BL21 under the control of T7 promoter with a high level of 70 and 12 mg protein per gram of wet cells, respectively. The simply purified lipase LipA was effectively refolded by Ni–NTA purified chaperone ΔLipBhis in molar ratio 1:1 at 4 °C for 24 hours in H2O. The in vitro refolded lipase LipA had an optimal activity in the temperature range of 50–55 °C and was stable up to 45 °C with more than 84% activity retention. The maximal activity was observed at pH 10.75 for hydrolysis of olive oil and found to be stable over alkaline pH range 8.0–10.5 with more than 52% activity retention. The enzyme was found to be highly resistant to many organic solvents especially induced by ethanolamine (remaining activity 137–334%), but inhibited by 1-butanol and acetonitrile (40–86%). Metal ions Cu2+, Sn2+, Mn2+, Mg2+, and Ca2+ stimulated the lipase slightly with increase in activity by up to 22%, whereas Zn2+ significantly inhibited the enzyme with the residual activity of 30–65% and Fe3+ to a lesser degree (activity retention of 77–86%). Tween 80, Tween 60, and Tween 40 induced the activation of the lipase LipA (222–330%) and 0.2–1% (w/v) of Triton X-100, X-45, and SDS increased the lipase activity by up to 52%. However, 5% (w/v) of Triton X-100, X-45, and SDS inhibited strongly the activity by 31–89%. The inhibitors including DEPC, EDTA, PMSF, and 2-mercaptoethanol (0.1–10 mM) inhibited moderately the lipase with remaining activity of 57–105%. The lipase LipA hydrolyzed a wide range of triglycerides, but preferentially short length acyl chains (C4 and C6). In contrast to the triglycerides, medium length acyl chains (C8 and C14) of p-nitrophenyl (p-NP) esters were preferential substrates of this lipase. The enzyme preferentially catalyzed the hydrolysis of cottonseed oil (317%), cornoil (227%), palm oil (222%), and wheatgerm oil (210%) in comparison to olive oil (100%)." @default.
• W2017017620 created "2016-06-24" @default.
• W2017017620 creator A5022252228 @default.
• W2017017620 creator A5036685396 @default.
• W2017017620 creator A5058509539 @default.
• W2017017620 creator A5068100738 @default.
• W2017017620 creator A5069961707 @default.
• W2017017620 date "2005-01-01" @default.
• W2017017620 modified "2023-10-16" @default.
• W2017017620 title "High-level heterologous expression and properties of a novel lipase from Ralstonia sp. M1" @default.
• W2017017620 cites W1568913968 @default.
• W2017017620 cites W1723311567 @default.
• W2017017620 cites W1801314748 @default.
• W2017017620 cites W1970807039 @default.
• W2017017620 cites W1981546206 @default.
• W2017017620 cites W1982360462 @default.
• W2017017620 cites W2006091918 @default.
• W2017017620 cites W2019618958 @default.
• W2017017620 cites W2040551576 @default.
• W2017017620 cites W2049506201 @default.
• W2017017620 cites W2062767843 @default.
• W2017017620 cites W2077788536 @default.
• W2017017620 cites W2100837269 @default.
• W2017017620 cites W2108849482 @default.
• W2017017620 cites W2123703288 @default.
• W2017017620 cites W2126733189 @default.
• W2017017620 cites W2152884644 @default.
• W2017017620 cites W2168175347 @default.
• W2017017620 cites W4248052168 @default.
• W2017017620 doi "https://doi.org/10.1016/j.pep.2004.10.001" @default.
• W2017017620 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/15596365" @default.
• W2017017620 hasPublicationYear "2005" @default.
• W2017017620 type Work @default.
• W2017017620 sameAs 2017017620 @default.
• W2017017620 citedByCount "24" @default.
• W2017017620 countsByYear W20170176202012 @default.
• W2017017620 countsByYear W20170176202013 @default.
• W2017017620 countsByYear W20170176202014 @default.
• W2017017620 countsByYear W20170176202016 @default.
• W2017017620 countsByYear W20170176202017 @default.
• W2017017620 countsByYear W20170176202018 @default.
• W2017017620 countsByYear W20170176202020 @default.
• W2017017620 countsByYear W20170176202022 @default.
• W2017017620 crossrefType "journal-article" @default.
• W2017017620 hasAuthorship W2017017620A5022252228 @default.
• W2017017620 hasAuthorship W2017017620A5036685396 @default.
• W2017017620 hasAuthorship W2017017620A5058509539 @default.
• W2017017620 hasAuthorship W2017017620A5068100738 @default.
• W2017017620 hasAuthorship W2017017620A5069961707 @default.
• W2017017620 hasConcept C104317684 @default.
• W2017017620 hasConcept C13965031 @default.
• W2017017620 hasConcept C153911025 @default.
• W2017017620 hasConcept C181199279 @default.
• W2017017620 hasConcept C185592680 @default.
• W2017017620 hasConcept C198684833 @default.
• W2017017620 hasConcept C2779697368 @default.
• W2017017620 hasConcept C28212737 @default.
• W2017017620 hasConcept C43617362 @default.
• W2017017620 hasConcept C547475151 @default.
• W2017017620 hasConcept C55493867 @default.
• W2017017620 hasConcept C86803240 @default.
• W2017017620 hasConcept C94412978 @default.
• W2017017620 hasConceptScore W2017017620C104317684 @default.
• W2017017620 hasConceptScore W2017017620C13965031 @default.
• W2017017620 hasConceptScore W2017017620C153911025 @default.
• W2017017620 hasConceptScore W2017017620C181199279 @default.
• W2017017620 hasConceptScore W2017017620C185592680 @default.
• W2017017620 hasConceptScore W2017017620C198684833 @default.
• W2017017620 hasConceptScore W2017017620C2779697368 @default.
• W2017017620 hasConceptScore W2017017620C28212737 @default.
• W2017017620 hasConceptScore W2017017620C43617362 @default.
• W2017017620 hasConceptScore W2017017620C547475151 @default.
• W2017017620 hasConceptScore W2017017620C55493867 @default.
• W2017017620 hasConceptScore W2017017620C86803240 @default.
• W2017017620 hasConceptScore W2017017620C94412978 @default.
• W2017017620 hasIssue "1" @default.
• W2017017620 hasLocation W20170176201 @default.
• W2017017620 hasLocation W20170176202 @default.
• W2017017620 hasOpenAccess W2017017620 @default.
• W2017017620 hasPrimaryLocation W20170176201 @default.
• W2017017620 hasRelatedWork W1983381624 @default.
• W2017017620 hasRelatedWork W2013084152 @default.
• W2017017620 hasRelatedWork W2047856863 @default.
• W2017017620 hasRelatedWork W2094752049 @default.
• W2017017620 hasRelatedWork W2246782474 @default.
• W2017017620 hasRelatedWork W2370219981 @default.
• W2017017620 hasRelatedWork W2587948215 @default.
• W2017017620 hasRelatedWork W3134843034 @default.
• W2017017620 hasRelatedWork W4289920555 @default.
• W2017017620 hasRelatedWork W4324149490 @default.
• W2017017620 hasVolume "39" @default.
• W2017017620 isParatext "false" @default.
• W2017017620 isRetracted "false" @default.
• W2017017620 magId "2017017620" @default.
• W2017017620 workType "article" @default.