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- W2017018439 abstract "When affinity chromatography with lentil lectin (LcH)-Sepharose was carried out in 0.5% (w/v) sodium deoxycholate (DOC), 10 mm Tris, pH 8.2, LcH consistently appeared in the 0.1 M α-methyl-mannoside (α-MeMan)-eluted fraction. Eluted LcH was recovered in both the control and specific immunoprecipitates with antisera directed against components of the glycoprotein fraction, suggesting that at least a portion of the LcH retained the capacity to interact with protein-bound carbohydrate. We suggest that the presence of functional LcH in the purified glycoprotein fraction is a potential source of artifact which may not be generally appreciated. When the non-ionic detergent, Nonidet P-40, was substituted for DOC in the same type of LcH-Sepharose fractionation, LcH did not appear in the 0.1 M α-MeMan-eluted fraction." @default.
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- W2017018439 date "1982-01-01" @default.
- W2017018439 modified "2023-10-18" @default.
- W2017018439 title "A cautionary note on the use of lentil lectin affinity chromatography in the presence of sodium deoxycholate" @default.
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- W2017018439 doi "https://doi.org/10.1016/0022-1759(82)90213-7" @default.
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