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- W2017019874 abstract "The enzyme β-amylase (1,4-α-d-Glucan maltohydrolase, E.C. 3.2.1.2) was isolated and purified to homogenity from the extract of healthy radish roots (Raphanus sativus). The purification involved ammonium sulphate precipitation (100% saturation); DEAE-cellulose; hydroxylapatite and Sephadex G-200 chromatography. The purity and homogenity of the enzyme preparation were judged by gel filtration on Sephadex G-200 and disc electrophoresis.The amount of the original enzyme activity remaining was 23% after 195·2 times purification, with specific activity 820 U/mg protein. The enzyme was active against starch, glycogen and α-dextrin but it failed to hydrolyze sucrose, maltose and lactose. Its molecular weight was 58 880 daltons, as estimated by gel filtration on Sephadex G-200. The Km value was 2·85% for soluble starch at optimum pH 5·0 and 45°C. The enzyme was relatively heat-stable for 15 min at 30 and 40°C, showing only about 8% loss of activity. The enzyme was completely inactivated by Cu+ 2, Fe+ 2, Ag+, Hg+ 2, but only moderately inhibited by p-chloromercuribenzoate. Strongly activated enzyme was obtained with EDTA, Zn+ 2, K+, Ca+ 2 and Co+ 2." @default.
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- W2017019874 date "1995-10-01" @default.
- W2017019874 modified "2023-10-17" @default.
- W2017019874 title "An experiment on the chemical modification of essential tryptophan residues of barley β-amylase" @default.
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- W2017019874 doi "https://doi.org/10.1016/0307-4412(95)00087-j" @default.
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