FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2017031407> ?p ?o ?g. }

• W2017031407 endingPage "49" @default.
• W2017031407 startingPage "43" @default.
• W2017031407 abstract "The marine flagellate Pavlova lutheri is a microalga known to be rich in long-chain polyunsaturated fatty acids (LC-PUFAs) and able to produce large amounts of n-3 fatty acids, such as eicosapentaenoic acid (EPA, 20:5n-3) and docosahexaenoic acid (DHA, 22:6n-3). As no previous study had attempted to measure the metabolic step of fatty acid synthesis in this alga, we used radiolabeled precursors to explore the various desaturation and elongation steps involved in LC-PUFA biosynthesis pathways. The incorporation of (14)C-labeled palmitic ([1-(14)C] 16:0) and dihomo-γ-linolenic ([1-(14)C] 20:3n-6) acids as ammonium salts within the cells was monitored during incubation periods lasting 3, 10 or 24h. Total lipids and each of the fatty acids were also monitored during these incubation periods. A decrease in the availability and/or accessibility of the radiolabeled substrates was observed over the incubation time. This decrease with incubation time observed using [1-(14)C] 16:0 and [1-(14)C] 20:3n-6 as substrates was used to monitor the conversion of (14)C-labeled arachidonic acid ([1-(14)C] 20:4n-6) into longer and more unsaturated fatty acids, such as 20:5n-3 and 22:6n-3, over shorter incubation times (1 and 3h). A metabolic relationship between the n-6 and n-3 fatty acid series was demonstrated in P. lutheri by measuring the Δ17-desaturation activity involved in the conversion of eicosatetraenoic acid to 20:5n-3. Our findings suggest that the biosynthesis pathway leading to n-3 LC-PUFA involves fatty acids of the n-6 family, which act as precursors in the biosynthesis of 20:5n-3 and 22:6n-3. This preliminary work provides a method for studying microalgal LC-PUFA biosynthesis pathways and desaturase and elongase activities in vivo using externally-radiolabeled fatty acid precursors as substrates. The use of the [1-(14)C] 20:4n-6 substrate also highlighted the relationships between the n-6 and the n-3 fatty acid series (e.g. Δ17-desaturation), and the final elongation and desaturation steps required for n-3 LC-PUFA formation in P. lutheri." @default.
• W2017031407 created "2016-06-24" @default.
• W2017031407 creator A5010772586 @default.
• W2017031407 creator A5031205390 @default.
• W2017031407 creator A5032602262 @default.
• W2017031407 creator A5085255882 @default.
• W2017031407 date "2013-06-01" @default.
• W2017031407 modified "2023-10-10" @default.
• W2017031407 title "Use of radiolabeled substrates to determine the desaturase and elongase activities involved in eicosapentaenoic acid and docosahexaenoic acid biosynthesis in the marine microalga Pavlova lutheri" @default.
• W2017031407 cites W1275357281 @default.
• W2017031407 cites W1486477932 @default.
• W2017031407 cites W1504719529 @default.
• W2017031407 cites W1583223387 @default.
• W2017031407 cites W1980984779 @default.
• W2017031407 cites W1986090956 @default.
• W2017031407 cites W1993866895 @default.
• W2017031407 cites W1996089807 @default.
• W2017031407 cites W2005534653 @default.
• W2017031407 cites W2007079264 @default.
• W2017031407 cites W2016093676 @default.
• W2017031407 cites W2026203662 @default.
• W2017031407 cites W2056904589 @default.
• W2017031407 cites W2057106181 @default.
• W2017031407 cites W2059311051 @default.
• W2017031407 cites W2069298006 @default.
• W2017031407 cites W2071873630 @default.
• W2017031407 cites W2075633659 @default.
• W2017031407 cites W2091243862 @default.
• W2017031407 cites W2095008390 @default.
• W2017031407 cites W2095536791 @default.
• W2017031407 cites W2103464952 @default.
• W2017031407 cites W2107154530 @default.
• W2017031407 cites W2112983788 @default.
• W2017031407 cites W2118302373 @default.
• W2017031407 cites W2139213776 @default.
• W2017031407 cites W2165189049 @default.
• W2017031407 cites W2167956793 @default.
• W2017031407 cites W2300552860 @default.
• W2017031407 cites W2320046304 @default.
• W2017031407 cites W4248167786 @default.
• W2017031407 doi "https://doi.org/10.1016/j.phytochem.2013.02.014" @default.
• W2017031407 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/23528573" @default.
• W2017031407 hasPublicationYear "2013" @default.
• W2017031407 type Work @default.
• W2017031407 sameAs 2017031407 @default.
• W2017031407 citedByCount "13" @default.
• W2017031407 countsByYear W20170314072013 @default.
• W2017031407 countsByYear W20170314072014 @default.
• W2017031407 countsByYear W20170314072015 @default.
• W2017031407 countsByYear W20170314072017 @default.
• W2017031407 countsByYear W20170314072018 @default.
• W2017031407 countsByYear W20170314072019 @default.
• W2017031407 countsByYear W20170314072021 @default.
• W2017031407 countsByYear W20170314072023 @default.
• W2017031407 crossrefType "journal-article" @default.
• W2017031407 hasAuthorship W2017031407A5010772586 @default.
• W2017031407 hasAuthorship W2017031407A5031205390 @default.
• W2017031407 hasAuthorship W2017031407A5032602262 @default.
• W2017031407 hasAuthorship W2017031407A5085255882 @default.
• W2017031407 hasConcept C181199279 @default.
• W2017031407 hasConcept C185592680 @default.
• W2017031407 hasConcept C19038510 @default.
• W2017031407 hasConcept C25642318 @default.
• W2017031407 hasConcept C2776091944 @default.
• W2017031407 hasConcept C2777171753 @default.
• W2017031407 hasConcept C2778078955 @default.
• W2017031407 hasConcept C2779936771 @default.
• W2017031407 hasConcept C543025807 @default.
• W2017031407 hasConcept C553450214 @default.
• W2017031407 hasConcept C55493867 @default.
• W2017031407 hasConcept C62231903 @default.
• W2017031407 hasConcept C86803240 @default.
• W2017031407 hasConceptScore W2017031407C181199279 @default.
• W2017031407 hasConceptScore W2017031407C185592680 @default.
• W2017031407 hasConceptScore W2017031407C19038510 @default.
• W2017031407 hasConceptScore W2017031407C25642318 @default.
• W2017031407 hasConceptScore W2017031407C2776091944 @default.
• W2017031407 hasConceptScore W2017031407C2777171753 @default.
• W2017031407 hasConceptScore W2017031407C2778078955 @default.
• W2017031407 hasConceptScore W2017031407C2779936771 @default.
• W2017031407 hasConceptScore W2017031407C543025807 @default.
• W2017031407 hasConceptScore W2017031407C553450214 @default.
• W2017031407 hasConceptScore W2017031407C55493867 @default.
• W2017031407 hasConceptScore W2017031407C62231903 @default.
• W2017031407 hasConceptScore W2017031407C86803240 @default.
• W2017031407 hasFunder F4320322890 @default.
• W2017031407 hasFunder F4320338366 @default.
• W2017031407 hasLocation W20170314071 @default.
• W2017031407 hasLocation W20170314072 @default.
• W2017031407 hasLocation W20170314073 @default.
• W2017031407 hasLocation W20170314074 @default.
• W2017031407 hasOpenAccess W2017031407 @default.
• W2017031407 hasPrimaryLocation W20170314071 @default.
• W2017031407 hasRelatedWork W186746711 @default.
• W2017031407 hasRelatedWork W1984929305 @default.
• W2017031407 hasRelatedWork W1990478119 @default.
• W2017031407 hasRelatedWork W2029389630 @default.
• W2017031407 hasRelatedWork W2110419883 @default.

• next