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- W2017042419 abstract "A rapid flat gel isoelectric focusing method has been developed for the determination of apolipoprotein E phenotypes. Isoelectric focusing in 5% polyacrylamide flat gel with 8 mol/l urea and 2.8% pharmalyte (pH 4–6.5) was carried out at 3,000 V and 4°C for 1 h under a constant power of 30 W. The separation of apolipoprotein E isoproteins was good and the isoelectric points were determined. Using this method, the apolipoprotein E phenotype frequency was examined in the Japanese population, and a higher frequency of phenotype E3/3 and a lower frequency of phenotype E3/2 were found in Japanese than those reported for the German, American or English population. In our focusing system the cut-off point of apolipoprotein E2/E3 ratio between the phenotype E3/3 and E3/2 was assumed to be approximately 0.9. These results indicate that this method may be useful for the determination of apolipoprotein E phenotypes." @default.
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- W2017042419 date "1985-06-01" @default.
- W2017042419 modified "2023-10-10" @default.
- W2017042419 title "A rapid flat gel isoelectric focusing method for the determination of apolipoprotein E phenotypes and its application" @default.
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- W2017042419 doi "https://doi.org/10.1016/0009-8981(85)90269-4" @default.
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