FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2017054454> ?p ?o ?g. }

• W2017054454 endingPage "817" @default.
• W2017054454 startingPage "811" @default.
• W2017054454 abstract "Abstract It has been suggested that excitotoxicity could be contributing to dopamine cell loss after methylphenylpyridinium ion (MPP + ) exposure, although the literature regarding this is contradictory. Given that in cell culture excitotoxicity has been reported to be dependent on culture age, we postulated that these discrepant results might be explained by a difference in developmental expression of N ‐methyl‐ D ‐aspartate (NMDA) receptors. To test this, mesencephalic cells were cultured and the number of dopaminergic neurons (tyrosine hydroxylase‐immunoreactive cells [TH‐IR] cells) expressing the NMDA R1 subunit (NR1) was determined using double‐label immunofluorescence microscopy. An increase in the percentage of TH‐IR cells expressing NR1 occurred over time in culture and this correlated with the toxicity of NMDA. At 7 days in vitro (DIV 7), only 17% ( n = 167 cells/4 experiments) of TH‐IR cells expressed NR1 and these cells were insensitive to NMDA toxicity. This increased to 80% ( n = 254 cells/6 experiments) by DIV 11 and cultures were now susceptible to NMDA‐induced injury. Cultures grown for either 7 or 11 days were treated for 48 hr with increasing concentrations of MPP + (0.5–20 μM) and the loss of dopaminergic neurons was determined by cell counting. Cultures at DIV 7 were more sensitive to MPP + than 11‐day‐old cultures (LD 50 = ∼0.75 μM vs. 15 μM, respectively). Co‐exposure to MK‐801 (5 μM) did not protect against MPP + toxicity in young cultures, but attenuated MPP + toxicity in the older cultures, becoming statistically significant at 20 μM MPP + . These data indicate that the activation of NMDA receptors is not required for, but can contribute to, MPP + ‐induced neurodegeneration of dopaminergic cells in culture. © 2003 Wiley‐Liss, Inc." @default.
• W2017054454 created "2016-06-24" @default.
• W2017054454 creator A5012317445 @default.
• W2017054454 creator A5062532967 @default.
• W2017054454 date "2003-07-31" @default.
• W2017054454 modified "2023-10-15" @default.
• W2017054454 title "Relationship between NMDA receptor expression and MPP+ toxicity in cultured dopaminergic cells" @default.
• W2017054454 cites W1483563853 @default.
• W2017054454 cites W1500211708 @default.
• W2017054454 cites W1601613466 @default.
• W2017054454 cites W1822777999 @default.
• W2017054454 cites W1900066504 @default.
• W2017054454 cites W1946523045 @default.
• W2017054454 cites W1947356649 @default.
• W2017054454 cites W1947475203 @default.
• W2017054454 cites W1968079469 @default.
• W2017054454 cites W1969800514 @default.
• W2017054454 cites W1975739117 @default.
• W2017054454 cites W1980800373 @default.
• W2017054454 cites W1981614010 @default.
• W2017054454 cites W1983951782 @default.
• W2017054454 cites W1989645955 @default.
• W2017054454 cites W1990215384 @default.
• W2017054454 cites W1992333288 @default.
• W2017054454 cites W1997243628 @default.
• W2017054454 cites W1998670757 @default.
• W2017054454 cites W2005984111 @default.
• W2017054454 cites W2009809272 @default.
• W2017054454 cites W2010024285 @default.
• W2017054454 cites W2019121368 @default.
• W2017054454 cites W2031538419 @default.
• W2017054454 cites W2034067256 @default.
• W2017054454 cites W2042632237 @default.
• W2017054454 cites W2043988673 @default.
• W2017054454 cites W2044779910 @default.
• W2017054454 cites W2044855560 @default.
• W2017054454 cites W2050643060 @default.
• W2017054454 cites W2052262213 @default.
• W2017054454 cites W2054851830 @default.
• W2017054454 cites W2055308573 @default.
• W2017054454 cites W2062530699 @default.
• W2017054454 cites W2066433054 @default.
• W2017054454 cites W2077260129 @default.
• W2017054454 cites W2090367785 @default.
• W2017054454 cites W2094645072 @default.
• W2017054454 cites W2094985550 @default.
• W2017054454 cites W2097513682 @default.
• W2017054454 cites W2098676615 @default.
• W2017054454 cites W2111065594 @default.
• W2017054454 cites W2143506799 @default.
• W2017054454 cites W2150648464 @default.
• W2017054454 cites W2150656456 @default.
• W2017054454 cites W2151388741 @default.
• W2017054454 cites W2159359085 @default.
• W2017054454 cites W2159688851 @default.
• W2017054454 cites W2161344190 @default.
• W2017054454 cites W2346759983 @default.
• W2017054454 cites W4244501671 @default.
• W2017054454 cites W4294359531 @default.
• W2017054454 cites W54933682 @default.
• W2017054454 doi "https://doi.org/10.1002/jnr.10732" @default.
• W2017054454 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/12949907" @default.
• W2017054454 hasPublicationYear "2003" @default.
• W2017054454 type Work @default.
• W2017054454 sameAs 2017054454 @default.
• W2017054454 citedByCount "4" @default.
• W2017054454 crossrefType "journal-article" @default.
• W2017054454 hasAuthorship W2017054454A5012317445 @default.
• W2017054454 hasAuthorship W2017054454A5062532967 @default.
• W2017054454 hasConcept C126322002 @default.
• W2017054454 hasConcept C134018914 @default.
• W2017054454 hasConcept C137183658 @default.
• W2017054454 hasConcept C153911025 @default.
• W2017054454 hasConcept C159167319 @default.
• W2017054454 hasConcept C170493617 @default.
• W2017054454 hasConcept C185592680 @default.
• W2017054454 hasConcept C202751555 @default.
• W2017054454 hasConcept C2781012912 @default.
• W2017054454 hasConcept C29730261 @default.
• W2017054454 hasConcept C513476851 @default.
• W2017054454 hasConcept C54355233 @default.
• W2017054454 hasConcept C55493867 @default.
• W2017054454 hasConcept C67018056 @default.
• W2017054454 hasConcept C71924100 @default.
• W2017054454 hasConcept C81885089 @default.
• W2017054454 hasConcept C86803240 @default.
• W2017054454 hasConcept C95444343 @default.
• W2017054454 hasConcept C98274493 @default.
• W2017054454 hasConceptScore W2017054454C126322002 @default.
• W2017054454 hasConceptScore W2017054454C134018914 @default.
• W2017054454 hasConceptScore W2017054454C137183658 @default.
• W2017054454 hasConceptScore W2017054454C153911025 @default.
• W2017054454 hasConceptScore W2017054454C159167319 @default.
• W2017054454 hasConceptScore W2017054454C170493617 @default.
• W2017054454 hasConceptScore W2017054454C185592680 @default.
• W2017054454 hasConceptScore W2017054454C202751555 @default.
• W2017054454 hasConceptScore W2017054454C2781012912 @default.
• W2017054454 hasConceptScore W2017054454C29730261 @default.

• next