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- W2017055915 abstract "Physarum polycephalum is a lower eukaryote belonging to the amoebozoa group of organisms that forms macroscopic, multinucleate plasmodial cells during its developmental cycle. Plasmodia can exit proliferative growth and differentiate by forming fruiting bodies containing mononucleate, haploid spores. This process, called sporulation, is controlled by starvation and visible light. To genetically dissect the regulatory control of the commitment to sporulation, we have isolated plasmodial mutants that are altered in the photocontrol of sporulation in a phenotypic screen of N ‐ethyl‐ N ‐nitrosourea ( ENU ) mutagenized cells. Several non‐sporulating mutants were analyzed by measuring the light‐induced change in the expression pattern of a set of 35 genes using Ge XP multiplex reverse transcription–polymerase chain reaction with RNA isolated from individual plasmodial cells. Mutants showed altered patterns of differentially regulated genes in response to light stimulation. Some genes clearly displayed pairwise correlation in terms of their expression level as measured in individual plasmodial cells. The pattern of pairwise correlation differed in various mutants, suggesting that different upstream regulators were disabled in the different mutants. We propose that patterns of pairwise correlation in gene expression might be useful to infer the underlying gene regulatory network." @default.
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- W2017055915 date "2013-01-25" @default.
- W2017055915 modified "2023-10-17" @default.
- W2017055915 title "<i>Physarum polycephalum</i>mutants in the photocontrol of sporulation display altered patterns in the correlated expression of developmentally regulated genes" @default.
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- W2017055915 doi "https://doi.org/10.1111/dgd.12029" @default.
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