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- W2017065879 abstract "A method for the quantitative estimation of thyroglobulin iodoamino acid residues is described. It combines (1) digestion of thyroglobulin (or iodinated proteins) with pronase (10% by weight for 48 hr) and leucylaminopeptidase (20% by weight for 24 hr), (2) separation of the free iodoamino acids liberated by chromatography on a cation-exchange resin column (Dowex 50-X4) and their quantitative estimation by the sodium arsenite/ceric sulfate reaction operated automatically according to a modification of the procedure of Block and Mandl. The complete release of iodotyrosines and iodothyronines is obtained in the case of man and horse thyroglobulins. For other thyroglobulins (rat, sheep, ox, dog) undigested iodinated material represents between 3 and 6% of total iodine. Deiodination is low and never exceeds around 3% of total protein iodine. The procedure described in this paper definitely improves procedures that are already known." @default.
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- W2017065879 title "Thyroglobulin iodoamino acids estimation after digestion with pronase and leucylaminopeptidase" @default.
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- W2017065879 doi "https://doi.org/10.1016/0003-2697(70)90301-5" @default.
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