FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2017075227> ?p ?o ?g. }

• W2017075227 endingPage "e1005088" @default.
• W2017075227 startingPage "e1005088" @default.
• W2017075227 abstract "MHC class II (MHCII) genes are transactivated by the NOD-like receptor (NLR) family member CIITA, which is recruited to SXY enhancers of MHCII promoters via a DNA-binding enhanceosome complex. NLRC5, another NLR protein, was recently found to control transcription of MHC class I (MHCI) genes. However, detailed understanding of NLRC5's target gene specificity and mechanism of action remained lacking. We performed ChIP-sequencing experiments to gain comprehensive information on NLRC5-regulated genes. In addition to classical MHCI genes, we exclusively identified novel targets encoding non-classical MHCI molecules having important functions in immunity and tolerance. ChIP-sequencing performed with Rfx5(-/-) cells, which lack the pivotal enhanceosome factor RFX5, demonstrated its strict requirement for NLRC5 recruitment. Accordingly, Rfx5-knockout mice phenocopy Nlrc5 deficiency with respect to defective MHCI expression. Analysis of B cell lines lacking RFX5, RFXAP, or RFXANK further corroborated the importance of the enhanceosome for MHCI expression. Although recruited by common DNA-binding factors, CIITA and NLRC5 exhibit non-redundant functions, shown here using double-deficient Nlrc5(-/-)CIIta(-/-) mice. These paradoxical findings were resolved by using a de novo motif-discovery approach showing that the SXY consensus sequence occupied by NLRC5 in vivo diverges significantly from that occupied by CIITA. These sequence differences were sufficient to determine preferential occupation and transactivation by NLRC5 or CIITA, respectively, and the S box was found to be the essential feature conferring NLRC5 specificity. These results broaden our knowledge on the transcriptional activities of NLRC5 and CIITA, revealing their dependence on shared enhanceosome factors but their recruitment to distinct enhancer motifs in vivo. Furthermore, we demonstrated selectivity of NLRC5 for genes encoding MHCI or related proteins, rendering it an attractive target for therapeutic intervention. NLRC5 and CIITA thus emerge as paradigms for a novel class of transcriptional regulators dedicated for transactivating extremely few, phylogenetically related genes." @default.
• W2017075227 created "2016-06-24" @default.
• W2017075227 creator A5000833036 @default.
• W2017075227 creator A5011824437 @default.
• W2017075227 creator A5017520558 @default.
• W2017075227 creator A5027201117 @default.
• W2017075227 creator A5040955861 @default.
• W2017075227 creator A5058535088 @default.
• W2017075227 creator A5062328451 @default.
• W2017075227 creator A5072397320 @default.
• W2017075227 creator A5074803235 @default.
• W2017075227 creator A5085859037 @default.
• W2017075227 date "2015-03-26" @default.
• W2017075227 modified "2023-10-07" @default.
• W2017075227 title "NLRC5 Exclusively Transactivates MHC Class I and Related Genes through a Distinctive SXY Module" @default.
• W2017075227 cites W1519266993 @default.
• W2017075227 cites W1629239351 @default.
• W2017075227 cites W1669971307 @default.
• W2017075227 cites W1920211008 @default.
• W2017075227 cites W1947203310 @default.
• W2017075227 cites W1956285448 @default.
• W2017075227 cites W1967139098 @default.
• W2017075227 cites W1968821076 @default.
• W2017075227 cites W1973051874 @default.
• W2017075227 cites W1986783333 @default.
• W2017075227 cites W1994569001 @default.
• W2017075227 cites W1995744312 @default.
• W2017075227 cites W2013255473 @default.
• W2017075227 cites W2015450090 @default.
• W2017075227 cites W2018185988 @default.
• W2017075227 cites W2028540377 @default.
• W2017075227 cites W2038941406 @default.
• W2017075227 cites W2045062204 @default.
• W2017075227 cites W2045474542 @default.
• W2017075227 cites W2053847949 @default.
• W2017075227 cites W2064360143 @default.
• W2017075227 cites W2065703299 @default.
• W2017075227 cites W2071287590 @default.
• W2017075227 cites W2080286449 @default.
• W2017075227 cites W2082389503 @default.
• W2017075227 cites W2097056602 @default.
• W2017075227 cites W2107922733 @default.
• W2017075227 cites W2111211467 @default.
• W2017075227 cites W2118557165 @default.
• W2017075227 cites W2120957859 @default.
• W2017075227 cites W2123504767 @default.
• W2017075227 cites W2124514674 @default.
• W2017075227 cites W2124985265 @default.
• W2017075227 cites W2134764584 @default.
• W2017075227 cites W2144654387 @default.
• W2017075227 cites W2147549042 @default.
• W2017075227 cites W2159193108 @default.
• W2017075227 cites W2172065556 @default.
• W2017075227 cites W2403341971 @default.
• W2017075227 doi "https://doi.org/10.1371/journal.pgen.1005088" @default.
• W2017075227 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4374748" @default.
• W2017075227 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/25811463" @default.
• W2017075227 hasPublicationYear "2015" @default.
• W2017075227 type Work @default.
• W2017075227 sameAs 2017075227 @default.
• W2017075227 citedByCount "72" @default.
• W2017075227 countsByYear W20170752272016 @default.
• W2017075227 countsByYear W20170752272017 @default.
• W2017075227 countsByYear W20170752272018 @default.
• W2017075227 countsByYear W20170752272019 @default.
• W2017075227 countsByYear W20170752272020 @default.
• W2017075227 countsByYear W20170752272021 @default.
• W2017075227 countsByYear W20170752272022 @default.
• W2017075227 countsByYear W20170752272023 @default.
• W2017075227 crossrefType "journal-article" @default.
• W2017075227 hasAuthorship W2017075227A5000833036 @default.
• W2017075227 hasAuthorship W2017075227A5011824437 @default.
• W2017075227 hasAuthorship W2017075227A5017520558 @default.
• W2017075227 hasAuthorship W2017075227A5027201117 @default.
• W2017075227 hasAuthorship W2017075227A5040955861 @default.
• W2017075227 hasAuthorship W2017075227A5058535088 @default.
• W2017075227 hasAuthorship W2017075227A5062328451 @default.
• W2017075227 hasAuthorship W2017075227A5072397320 @default.
• W2017075227 hasAuthorship W2017075227A5074803235 @default.
• W2017075227 hasAuthorship W2017075227A5085859037 @default.
• W2017075227 hasBestOaLocation W20170752271 @default.
• W2017075227 hasConcept C104317684 @default.
• W2017075227 hasConcept C1292079 @default.
• W2017075227 hasConcept C170627219 @default.
• W2017075227 hasConcept C207936829 @default.
• W2017075227 hasConcept C2775890610 @default.
• W2017075227 hasConcept C2778814158 @default.
• W2017075227 hasConcept C54355233 @default.
• W2017075227 hasConcept C86339819 @default.
• W2017075227 hasConcept C86803240 @default.
• W2017075227 hasConcept C95444343 @default.
• W2017075227 hasConceptScore W2017075227C104317684 @default.
• W2017075227 hasConceptScore W2017075227C1292079 @default.
• W2017075227 hasConceptScore W2017075227C170627219 @default.
• W2017075227 hasConceptScore W2017075227C207936829 @default.
• W2017075227 hasConceptScore W2017075227C2775890610 @default.
• W2017075227 hasConceptScore W2017075227C2778814158 @default.
• W2017075227 hasConceptScore W2017075227C54355233 @default.

• next