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- W2017089314 abstract "A novel DNA methylation assay technique, termed bisulfite single-strand conformation polymorphism (bisulfite-SSCP), is a combination of sodium-bisulfite modification and fluorescence-based polymerase chain reaction (PCR)-SSCP. After bisulfite treatment followed by PCR amplification, methylated and unmethylated alleles can be simultaneously separated in a nondenaturing gel using an automated DNA sequencer. Using bisulfite-SSCP, methylation of hMLH1 was detected in a quantitative manner. This method is not only simple, quick, accurate, and quantitative, but detailed information about methylation is also available with less work. Methylation analysis of large numbers of samples for multiple loci will be facilitated by bisulfite-SSCP." @default.
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- W2017089314 date "2000-03-01" @default.
- W2017089314 modified "2023-10-11" @default.
- W2017089314 title "Quantitative DNA methylation analysis by fluorescent polymerase chain reaction single-strand conformation polymorphism using an automated DNA sequencer" @default.
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- W2017089314 doi "https://doi.org/10.1002/(sici)1522-2683(20000301)21:5<904::aid-elps904>3.0.co;2-4" @default.
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