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- W2017100166 abstract "Glucocorticoid hormones promoted the growth of fibroblast cells derived from human neonatal foreskins and prolonged their life span in a microcarrier culture system that used Eagle's minimum essential medium (MEM) supplemented with fetal calf serum (FCS). But, these hormones suppressed cell growth in conventional monolayer cultures.Precolostrum newborn calf serum (PNCS) was the only species that sup-ported the serial propagation of fibroblast cells on microcarriers, possibly because of its high content of hydrocortisone (HC).Fibroblast cells grown on microcarriers in the presence of glucocorticoid hormones maintained their ability to produce interferon (IFN)-β in a super-induction method with poly I : poly C and antimetabolites. These cells had more than 93 % diploidy and no chromosomal aberration or translocation.Use of PNCS for the cultivation of human fibroblast cells has high potential for providing a microcarrier culture system for the mass production of human IFN-β." @default.
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- W2017100166 title "Effect of glucocorticoid hormones on growth of human fibroblast cells and interferon production in a microcarrier culture system." @default.
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