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- W2017101370 endingPage "560" @default.
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- W2017101370 abstract "ThePichia pastoris expression system has been successfully used for production of various recombinant heterogeneous proteins. The productivity ofP. pastoris can be improved substantially by bioreactor cultivations. However, heterologous proteins degradation increases as well in high-cell density culture. Proteolytic degradation is a serious problem since the yeast has been employed to express recombinant proteins. In this review, some of the recent developments, as well as strategies for reducing proteolytic degradation of the expressed recombinant protein at cultivation, cellular and protein levels on the cytosolic proteasome, vacuolar proteases, and proteases located within the secretory pathway inP. pastoris, are reviewed." @default.
- W2017101370 created "2016-06-24" @default.
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- W2017101370 creator A5021561750 @default.
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- W2017101370 date "2007-12-01" @default.
- W2017101370 modified "2023-10-07" @default.
- W2017101370 title "The proteolytic systems and heterologous proteins degradation in the methylotrophic yeastPichia pastoris" @default.
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- W2017101370 doi "https://doi.org/10.1007/bf03175354" @default.
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