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- W2017103445 abstract "The present report describes a simple, rapid and reproducible procedure for preparation of hepatitis B surface antigen from human positive sera, with a satisfactory yield. After removal of β- and α-lipoproteins by dextran sulphate precipitation and subsequent removal of IgM and a part of the other serum proteins by dialysis against distilled water, the supernatant obtained presents almost no change in its surface antigen content. After passing this solution through a molecular sieve of Biogel A-5 m, the surface antigen is detected in a well-defined peak. The fraction corresponding to this peak is collected in its totality. After lyophilization, it is passed through a molecular sieve of CPG 10, in phosphate buffer; this allows recovery of a substance which has retained its structural as well as its antigenic properties, and which is intact and practically free of any serum contamination." @default.
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- W2017103445 date "1977-01-01" @default.
- W2017103445 modified "2023-09-25" @default.
- W2017103445 title "Methodologie simple de separation et de purification de l'antigene de surface de l'hepatite B a partir du serum" @default.
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- W2017103445 doi "https://doi.org/10.1016/0009-8981(77)90388-6" @default.
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