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• W2017110746 abstract "Many bacteria utilize riboswitch transcription regulation to monitor and appropriately respond to cellular levels of important metabolites or effector molecules. The T box transcription antitermination riboswitch responds to cognate uncharged tRNA by specifically stabilizing an antiterminator element in the 5′-untranslated mRNA leader region and precluding formation of a thermodynamically more stable terminator element. Stabilization occurs when the tRNA acceptor end base pairs with the first four nucleotides in the seven nucleotide bulge of the highly conserved antiterminator element. The significance of the conservation of the antiterminator bulge nucleotides that do not base pair with the tRNA is unknown, but they are required for optimal function. In vitro selection was used to determine if the isolated antiterminator bulge context alone dictates the mode in which the tRNA acceptor end binds the bulge nucleotides. No sequence conservation beyond complementarity was observed and the location was not constrained to the first four bases of the bulge. The results indicate that formation of a structure that recognizes the tRNA acceptor end in isolation is not the determinant driving force for the high phylogenetic sequence conservation observed within the antiterminator bulge. Additional factors or T box leader features more likely influenced the phylogenetic sequence conservation." @default.
• W2017110746 created "2016-06-24" @default.
• W2017110746 creator A5011329147 @default.
• W2017110746 creator A5032065932 @default.
• W2017110746 creator A5090257969 @default.
• W2017110746 date "2009-03-01" @default.
• W2017110746 modified "2023-09-27" @default.
• W2017110746 title "T box transcription antitermination riboswitch: Influence of nucleotide sequence and orientation on tRNA binding by the antiterminator element" @default.
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• W2017110746 doi "https://doi.org/10.1016/j.bbagrm.2008.12.004" @default.
• W2017110746 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2656570" @default.
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