FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2017112676> ?p ?o ?g. }

• W2017112676 endingPage "609" @default.
• W2017112676 startingPage "600" @default.
• W2017112676 abstract "Background Volatile anesthetics have been shown to modify immune cell functions via several mechanisms, some of which have been only partially elucidated. We demonstrated that isoflurane inhibits primary leukocyte integrin lymphocyte function-associated antigen-1 (LFA-1) by binding to the allosteric cavity critical for conformational activation to its high-affinity form. It remains to be determined whether the allosteric inhibition of LFA-1 by isoflurane can be generalized to other anesthetics such as sevoflurane. Methods The effects of sevoflurane on the ability of LFA-1 to bind to its counter-ligand, intercellular adhesion molecule-1, was studied in leukocytes by flow cytometry. To examine whether sevoflurane acts directly on LFA-1, we measured ligand-binding using beads coated with purified LFA-1 protein. To distinguish between competitive versus allosteric inhibition, we analyzed the effects of sevoflurane on both wild-type and mutant-locked high-affinity LFA-1. One-way analysis of variance was employed for statistical analysis of the data. Nuclear magnetic resonance spectroscopy was used to identify sevoflurane binding site(s). Results Sevoflurane at clinically relevant concentrations inhibited the ligand-binding function of LFA-1 in leukocytes as well as in cell-free assays (P&lt;0.05). Sevoflurane blocked wild-type but not locked high-affinity LFA-1, thereby demonstrating an allosteric mode of inhibition. Nuclear magnetic resonance spectroscopy revealed that sevoflurane bound to the allosteric cavity, to which LFA-1 allosteric antagonists and isoflurane also bind. Conclusions This study suggests that sevoflurane also blocks the activation-dependent conformational changes of LFA-1 to the high-affinity form. The allosteric mode of action exemplified by sevoflurane and isoflurane via LFA-1 might represent one of the underlying mechanisms of anesthetic-mediated immunomodulation." @default.
• W2017112676 created "2016-06-24" @default.
• W2017112676 creator A5015363564 @default.
• W2017112676 creator A5019087762 @default.
• W2017112676 creator A5053036995 @default.
• W2017112676 creator A5070196487 @default.
• W2017112676 creator A5086932565 @default.
• W2017112676 date "2010-09-01" @default.
• W2017112676 modified "2023-10-18" @default.
• W2017112676 title "Sevoflurane Binds and Allosterically Blocks Integrin Lymphocyte Function-associated Antigen-1" @default.
• W2017112676 cites W1479880028 @default.
• W2017112676 cites W1516877092 @default.
• W2017112676 cites W1576773659 @default.
• W2017112676 cites W1840025613 @default.
• W2017112676 cites W193148329 @default.
• W2017112676 cites W1963577615 @default.
• W2017112676 cites W1965695253 @default.
• W2017112676 cites W1967111434 @default.
• W2017112676 cites W1968177806 @default.
• W2017112676 cites W1975649958 @default.
• W2017112676 cites W1976210308 @default.
• W2017112676 cites W1978348024 @default.
• W2017112676 cites W1991964259 @default.
• W2017112676 cites W1996880045 @default.
• W2017112676 cites W1999423218 @default.
• W2017112676 cites W2003285993 @default.
• W2017112676 cites W2013546235 @default.
• W2017112676 cites W2015313829 @default.
• W2017112676 cites W2019502698 @default.
• W2017112676 cites W2024383876 @default.
• W2017112676 cites W2025237036 @default.
• W2017112676 cites W2058505658 @default.
• W2017112676 cites W2059196027 @default.
• W2017112676 cites W2063330553 @default.
• W2017112676 cites W2063559096 @default.
• W2017112676 cites W2069582829 @default.
• W2017112676 cites W2069602336 @default.
• W2017112676 cites W2075984992 @default.
• W2017112676 cites W2078907724 @default.
• W2017112676 cites W2079929548 @default.
• W2017112676 cites W2082814731 @default.
• W2017112676 cites W2085979652 @default.
• W2017112676 cites W2095272173 @default.
• W2017112676 cites W2098041943 @default.
• W2017112676 cites W2099828575 @default.
• W2017112676 cites W2103978105 @default.
• W2017112676 cites W2104159031 @default.
• W2017112676 cites W2109290491 @default.
• W2017112676 cites W2109319033 @default.
• W2017112676 cites W2111620202 @default.
• W2017112676 cites W2115769413 @default.
• W2017112676 cites W2125435732 @default.
• W2017112676 cites W2133169381 @default.
• W2017112676 cites W2133609727 @default.
• W2017112676 cites W2150744739 @default.
• W2017112676 cites W2154003559 @default.
• W2017112676 cites W2158810433 @default.
• W2017112676 cites W2159721819 @default.
• W2017112676 cites W2159732031 @default.
• W2017112676 cites W2169821755 @default.
• W2017112676 cites W2170816379 @default.
• W2017112676 cites W2170877356 @default.
• W2017112676 cites W2313422370 @default.
• W2017112676 cites W2319396397 @default.
• W2017112676 cites W2332870608 @default.
• W2017112676 cites W279578989 @default.
• W2017112676 cites W66079651 @default.
• W2017112676 doi "https://doi.org/10.1097/aln.0b013e3181e89a77" @default.
• W2017112676 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/5209785" @default.
• W2017112676 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/20693879" @default.
• W2017112676 hasPublicationYear "2010" @default.
• W2017112676 type Work @default.
• W2017112676 sameAs 2017112676 @default.
• W2017112676 citedByCount "56" @default.
• W2017112676 countsByYear W20171126762012 @default.
• W2017112676 countsByYear W20171126762013 @default.
• W2017112676 countsByYear W20171126762014 @default.
• W2017112676 countsByYear W20171126762015 @default.
• W2017112676 countsByYear W20171126762016 @default.
• W2017112676 countsByYear W20171126762017 @default.
• W2017112676 countsByYear W20171126762018 @default.
• W2017112676 countsByYear W20171126762019 @default.
• W2017112676 countsByYear W20171126762020 @default.
• W2017112676 countsByYear W20171126762021 @default.
• W2017112676 countsByYear W20171126762023 @default.
• W2017112676 crossrefType "journal-article" @default.
• W2017112676 hasAuthorship W2017112676A5015363564 @default.
• W2017112676 hasAuthorship W2017112676A5019087762 @default.
• W2017112676 hasAuthorship W2017112676A5053036995 @default.
• W2017112676 hasAuthorship W2017112676A5070196487 @default.
• W2017112676 hasAuthorship W2017112676A5086932565 @default.
• W2017112676 hasBestOaLocation W20171126761 @default.
• W2017112676 hasConcept C116569031 @default.
• W2017112676 hasConcept C12554922 @default.
• W2017112676 hasConcept C1491633281 @default.
• W2017112676 hasConcept C166342909 @default.
• W2017112676 hasConcept C170493617 @default.
• W2017112676 hasConcept C185592680 @default.

• next