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- W2017113354 abstract "The amino acid composition was determined directly from protein bands from Amido Schwarz stained polyacrylamide gels. Tyrosine is completely destroyed and histidine partially destroyed upon hydrolysis. This could be prevented by the inclusion of β-mercaptoethanol during hydrolysis. A 15 cm column was required for analysis of the basic residues in order to prevent overlap between the large amounts of ammonia produced and histidine. Bovine serum albumin, ovalbumin, and two isozymes of beef heart lactate dehydrogenase were analyzed after gel electrophoresis, staining, and destaining. It was shown that good accuracy and reproducibility can be attained using this method. The method should help in the analysis of isozymes and in determining the composition of subunits separated under dissociating conditions." @default.
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- W2017113354 date "1971-11-01" @default.
- W2017113354 modified "2023-09-26" @default.
- W2017113354 title "Amino acid analysis of stained bands from polyacrylamide gels" @default.
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- W2017113354 doi "https://doi.org/10.1016/0003-2697(71)90348-4" @default.
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