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- W2017123428 abstract "Six different serological methods were tested for their ability to diagnose human hydatidosis. These were indirect immunofluorescence, indirect hemagglutination, counter immunoelectrophoresis and enzyme-linked immunosorbent assay (ELISA). In all, 298 sera were examined: 151 sera from negative control subjects, 78 sera from patients with proven hydatidosis, and 69 sera from patients with disease not due to hydatidosis. The last group of samples were used to define the technique's specificity. The specificity and sensitivity of the two recent techniques: enzyme-linked immunoelectrodiffusion assay (ELIEDA) and immunoblotting (electrophoretic migration under denatured and reducing conditions of hydatid fluid antigens, blot and immunoenzymatic assay) were tested mainly on doubtful or weakly positive sera from patients with proven hydatidosis and with filariasis. The combination of enzymelinked immunosorbent assay, indirect immunofluorescence and counter immunoelectrophoresis or ELISA and ELIEDA gave the most dependable and reliable serodiagnostic results with our antigens. ELIEDA is the most useful technique if only one can be used. Our preliminary results with ELIEDA confirm the good specificity and sensitivity of this technique and allow, in some cases, an interpretation of false positive values obtained with ELISA and indirect immunofluorescence. This method also confirms, in most cases, the disease by revealing the arc 5 in patients with low specific IgG content. Immunoblotting in our experimental conditions has a lower sensitivity than ELIEDA but has a specificity for certain proteins (10–12, 15–18, 220, 230 kDa)." @default.
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- W2017123428 date "1989-08-01" @default.
- W2017123428 modified "2023-10-18" @default.
- W2017123428 title "The immunodiagnostic value of six serological techniques in hydatidosis" @default.
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- W2017123428 doi "https://doi.org/10.1016/0888-0786(89)90003-6" @default.
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