FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2017125864> ?p ?o ?g. }

• W2017125864 endingPage "20867" @default.
• W2017125864 startingPage "20856" @default.
• W2017125864 abstract "Here we performed structural and biochemical analyses on the TK2285 gene product, an uncharacterized protein annotated as a member of the ribokinase family, from the hyperthermophilic archaeon Thermococcus kodakarensis. The three-dimensional structure of the TK2285 protein resembled those of previously characterized members of the ribokinase family including ribokinase, adenosine kinase, and phosphofructokinase. Conserved residues characteristic of this protein family were located in a cleft of the TK2285 protein as in other members whose structures have been determined. We thus examined the kinase activity of the TK2285 protein toward various sugars recognized by well characterized ribokinase family members. Although activity with sugar phosphates and nucleosides was not detected, kinase activity was observed toward d-allose, d-lyxose, d-tagatose, d-talose, d-xylose, and d-xylulose. Kinetic analyses with the six sugar substrates revealed high Km values, suggesting that they were not the true physiological substrates. By examining activity toward amino sugars, sugar alcohols, and disaccharides, we found that the TK2285 protein exhibited prominent kinase activity toward myo-inositol. Kinetic analyses with myo-inositol revealed a greater kcat and much lower Km value than those obtained with the monosaccharides, resulting in over a 2,000-fold increase in kcat/Km values. TK2285 homologs are distributed among members of Thermococcales, and in most species, the gene is positioned close to a myo-inositol monophosphate synthase gene. Our results suggest the presence of a novel subfamily of the ribokinase family whose members are present in Archaea and recognize myo-inositol as a substrate." @default.
• W2017125864 created "2016-06-24" @default.
• W2017125864 creator A5003219086 @default.
• W2017125864 creator A5019050913 @default.
• W2017125864 creator A5020165491 @default.
• W2017125864 creator A5047238739 @default.
• W2017125864 creator A5049138769 @default.
• W2017125864 creator A5052254225 @default.
• W2017125864 creator A5062941634 @default.
• W2017125864 creator A5069229339 @default.
• W2017125864 date "2013-07-01" @default.
• W2017125864 modified "2023-10-12" @default.
• W2017125864 title "An Uncharacterized Member of the Ribokinase Family in Thermococcus kodakarensis Exhibits myo-Inositol Kinase Activity" @default.
• W2017125864 cites W1501831169 @default.
• W2017125864 cites W1539796472 @default.
• W2017125864 cites W1563363363 @default.
• W2017125864 cites W1587133637 @default.
• W2017125864 cites W1971277373 @default.
• W2017125864 cites W1984878600 @default.
• W2017125864 cites W1989872945 @default.
• W2017125864 cites W1999364342 @default.
• W2017125864 cites W1999590520 @default.
• W2017125864 cites W2000497556 @default.
• W2017125864 cites W2001641653 @default.
• W2017125864 cites W2007158632 @default.
• W2017125864 cites W2013392613 @default.
• W2017125864 cites W2030517878 @default.
• W2017125864 cites W2034599009 @default.
• W2017125864 cites W2035319179 @default.
• W2017125864 cites W2035503835 @default.
• W2017125864 cites W2040048782 @default.
• W2017125864 cites W2055153067 @default.
• W2017125864 cites W2055881650 @default.
• W2017125864 cites W2057601066 @default.
• W2017125864 cites W2073233549 @default.
• W2017125864 cites W2073354998 @default.
• W2017125864 cites W2076710561 @default.
• W2017125864 cites W2077621660 @default.
• W2017125864 cites W2077875794 @default.
• W2017125864 cites W2079204867 @default.
• W2017125864 cites W2084738041 @default.
• W2017125864 cites W2088445270 @default.
• W2017125864 cites W2088678949 @default.
• W2017125864 cites W2092085661 @default.
• W2017125864 cites W2092092457 @default.
• W2017125864 cites W2095570870 @default.
• W2017125864 cites W2103722580 @default.
• W2017125864 cites W2109425768 @default.
• W2017125864 cites W2117819220 @default.
• W2017125864 cites W2120133190 @default.
• W2017125864 cites W2121404986 @default.
• W2017125864 cites W2121502968 @default.
• W2017125864 cites W2122339645 @default.
• W2017125864 cites W2124026197 @default.
• W2017125864 cites W2124279235 @default.
• W2017125864 cites W2126118592 @default.
• W2017125864 cites W2127594598 @default.
• W2017125864 cites W2132729882 @default.
• W2017125864 cites W2134984312 @default.
• W2017125864 cites W2138956739 @default.
• W2017125864 cites W2145206794 @default.
• W2017125864 cites W2166374078 @default.
• W2017125864 cites W2253429324 @default.
• W2017125864 doi "https://doi.org/10.1074/jbc.m113.457259" @default.
• W2017125864 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3774357" @default.
• W2017125864 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/23737529" @default.
• W2017125864 hasPublicationYear "2013" @default.
• W2017125864 type Work @default.
• W2017125864 sameAs 2017125864 @default.
• W2017125864 citedByCount "8" @default.
• W2017125864 countsByYear W20171258642014 @default.
• W2017125864 countsByYear W20171258642015 @default.
• W2017125864 countsByYear W20171258642017 @default.
• W2017125864 countsByYear W20171258642018 @default.
• W2017125864 countsByYear W20171258642019 @default.
• W2017125864 countsByYear W20171258642021 @default.
• W2017125864 crossrefType "journal-article" @default.
• W2017125864 hasAuthorship W2017125864A5003219086 @default.
• W2017125864 hasAuthorship W2017125864A5019050913 @default.
• W2017125864 hasAuthorship W2017125864A5020165491 @default.
• W2017125864 hasAuthorship W2017125864A5047238739 @default.
• W2017125864 hasAuthorship W2017125864A5049138769 @default.
• W2017125864 hasAuthorship W2017125864A5052254225 @default.
• W2017125864 hasAuthorship W2017125864A5062941634 @default.
• W2017125864 hasAuthorship W2017125864A5069229339 @default.
• W2017125864 hasBestOaLocation W20171258641 @default.
• W2017125864 hasConcept C104317684 @default.
• W2017125864 hasConcept C170493617 @default.
• W2017125864 hasConcept C181199279 @default.
• W2017125864 hasConcept C184235292 @default.
• W2017125864 hasConcept C2777427919 @default.
• W2017125864 hasConcept C2778899470 @default.
• W2017125864 hasConcept C2780535078 @default.
• W2017125864 hasConcept C550995028 @default.
• W2017125864 hasConcept C55493867 @default.
• W2017125864 hasConcept C86803240 @default.
• W2017125864 hasConcept C90934575 @default.
• W2017125864 hasConcept C94734110 @default.

• next