Matches in SemOpenAlex for { <https://semopenalex.org/work/W2017126936> ?p ?o ?g. }
- W2017126936 abstract "Signalling triggered by adhesion to the extracellular matrix plays a key role in the spatial orientation of epithelial polarity and formation of lumens in glandular tissues. Phosphoinositide 3-kinase signalling in particular is known to influence the polarization process during epithelial cell morphogenesis. Here, using Madin–Darby canine kidney epithelial cells grown in 3D culture, we show that the p110δ isoform of phosphoinositide 3-kinase co-localizes with focal adhesion proteins at the basal surface of polarized cells. Pharmacological, siRNA- or kinase-dead-mediated inhibition of p110δ impair the early stages of lumen formation, resulting in inverted polarized cysts, with no laminin or type IV collagen assembly at cell/extracellular matrix contacts. p110δ also regulates the organization of focal adhesions and membrane localization of dystroglycan. Thus, we uncover a previously unrecognized role for p110δ in epithelial cells in the orientation of the apico-basal axis and lumen formation. Kidney epithelial cells grown in 3D culture form polarized cysts in which basal membranes surround a lumen. Peng et al.show that the p110δ subunit of phosphoinositide 3-kinase contributes to the formation of these structures by influencing focal adhesion at the basal membrane." @default.
- W2017126936 created "2016-06-24" @default.
- W2017126936 creator A5002574488 @default.
- W2017126936 creator A5004123653 @default.
- W2017126936 creator A5030264520 @default.
- W2017126936 creator A5051010078 @default.
- W2017126936 creator A5059508363 @default.
- W2017126936 creator A5060003837 @default.
- W2017126936 creator A5061210699 @default.
- W2017126936 creator A5064057107 @default.
- W2017126936 creator A5071943800 @default.
- W2017126936 creator A5076973759 @default.
- W2017126936 creator A5087167240 @default.
- W2017126936 date "2015-01-13" @default.
- W2017126936 modified "2023-10-14" @default.
- W2017126936 title "Phosphoinositide 3-kinase p110δ promotes lumen formation through the enhancement of apico-basal polarity and basal membrane organization" @default.
- W2017126936 cites W1530052324 @default.
- W2017126936 cites W1541596409 @default.
- W2017126936 cites W1969570221 @default.
- W2017126936 cites W1972204116 @default.
- W2017126936 cites W1973113661 @default.
- W2017126936 cites W1974047237 @default.
- W2017126936 cites W1982818853 @default.
- W2017126936 cites W1983516907 @default.
- W2017126936 cites W1994824666 @default.
- W2017126936 cites W2001136908 @default.
- W2017126936 cites W2005800972 @default.
- W2017126936 cites W2005993913 @default.
- W2017126936 cites W2006628284 @default.
- W2017126936 cites W2009312865 @default.
- W2017126936 cites W2010980536 @default.
- W2017126936 cites W2016591815 @default.
- W2017126936 cites W2018440302 @default.
- W2017126936 cites W2019325963 @default.
- W2017126936 cites W2033352266 @default.
- W2017126936 cites W2036145275 @default.
- W2017126936 cites W2038444072 @default.
- W2017126936 cites W2038731722 @default.
- W2017126936 cites W2047385780 @default.
- W2017126936 cites W2047512850 @default.
- W2017126936 cites W2053256698 @default.
- W2017126936 cites W2054171565 @default.
- W2017126936 cites W2054530444 @default.
- W2017126936 cites W2056535053 @default.
- W2017126936 cites W2057733044 @default.
- W2017126936 cites W2058468502 @default.
- W2017126936 cites W2059053859 @default.
- W2017126936 cites W2062628051 @default.
- W2017126936 cites W2064982295 @default.
- W2017126936 cites W2066564017 @default.
- W2017126936 cites W2069354876 @default.
- W2017126936 cites W2070732456 @default.
- W2017126936 cites W2072765132 @default.
- W2017126936 cites W2076548587 @default.
- W2017126936 cites W2082416335 @default.
- W2017126936 cites W2087735787 @default.
- W2017126936 cites W2091884933 @default.
- W2017126936 cites W2091930538 @default.
- W2017126936 cites W2093720054 @default.
- W2017126936 cites W2095718486 @default.
- W2017126936 cites W2099468816 @default.
- W2017126936 cites W2101141712 @default.
- W2017126936 cites W2101244168 @default.
- W2017126936 cites W2106525346 @default.
- W2017126936 cites W2109410151 @default.
- W2017126936 cites W2115706649 @default.
- W2017126936 cites W2118342418 @default.
- W2017126936 cites W2120021279 @default.
- W2017126936 cites W2123666692 @default.
- W2017126936 cites W2124593509 @default.
- W2017126936 cites W2125519183 @default.
- W2017126936 cites W2127355894 @default.
- W2017126936 cites W2127388402 @default.
- W2017126936 cites W2130201056 @default.
- W2017126936 cites W2131717149 @default.
- W2017126936 cites W2134629247 @default.
- W2017126936 cites W2134861285 @default.
- W2017126936 cites W2135925002 @default.
- W2017126936 cites W2142820370 @default.
- W2017126936 cites W2143759076 @default.
- W2017126936 cites W2146734285 @default.
- W2017126936 cites W2152002380 @default.
- W2017126936 cites W2161930851 @default.
- W2017126936 cites W2163577434 @default.
- W2017126936 cites W2170349147 @default.
- W2017126936 cites W2330820039 @default.
- W2017126936 cites W39630652 @default.
- W2017126936 doi "https://doi.org/10.1038/ncomms6937" @default.
- W2017126936 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/5094449" @default.
- W2017126936 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/25583025" @default.
- W2017126936 hasPublicationYear "2015" @default.
- W2017126936 type Work @default.
- W2017126936 sameAs 2017126936 @default.
- W2017126936 citedByCount "35" @default.
- W2017126936 countsByYear W20171269362015 @default.
- W2017126936 countsByYear W20171269362016 @default.
- W2017126936 countsByYear W20171269362017 @default.
- W2017126936 countsByYear W20171269362018 @default.
- W2017126936 countsByYear W20171269362019 @default.
- W2017126936 countsByYear W20171269362020 @default.