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• W2017134691 abstract "Two strains of Entamoeba histolytica with pathogenic zymodemes (SFL3, HK9), one strain with non-pathogenic zymodeme (Bru) and one non-pathogenic Entamoeba sp. strain (cold strain), were investigated with respect to their interaction with target cells. Three test systems were used: 1) direct microscopical observation and qualitative as well as quantitative evaluation of contact and binding events with MDCK cells as targets, 2) kinetics of cytotoxic activity as measured by means of chromium release from 51Cr-labelled K562 cells, and 3) electrophysiological observations with freshly prepared mouse liver cells. We observed that the non-pathogenic cold strain interacted only shortly with target cells (statistical events, interaction type I), but did not induce morphological changes, chromium release or depolarization of targets. Non-pathogenic and avirulent strain Bru showed, apart from type I-binding, the ability to establish tight (type II) and long-lasting contact (type III) with targets, but again without cytotoxic effects. The pathogenic but avirulent strain HK9 tightly interacted (type II) and sometimes long-lasting with target cells, but morphological changes and chromium release were of a moderate degree during the first 20 min, and depolarization was only a rare event. In contrast, strain SFL3 produced tight and long-lasting contacts (type III binding), leading to cell death in 83% (type IV interaction) within 20 min, substantial chromium release within 10 min and rapid depolarization (electric collapse) of target cells." @default.
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• W2017134691 date "1989-06-01" @default.
• W2017134691 modified "2023-09-27" @default.
• W2017134691 title "Interaction of Different Strains of Entamoeba histolytica with Target Cells: Characterization of Electrophysiological and Morphological Features" @default.
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• W2017134691 doi "https://doi.org/10.1016/s0171-2985(89)80016-6" @default.
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