FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2017150501> ?p ?o ?g. }

• W2017150501 endingPage "6003" @default.
• W2017150501 startingPage "5993" @default.
• W2017150501 abstract "R6K-encoded pi protein can bind to the seven, 22 bp tandem iterons of the gamma origin. In this work, we use a variant of pi, His-pi.F107S, that is hyperactive in replication. In vitro, His-pi.F107S-dependent local DNA melting (open complex formation) occurs in the absence of host proteins (IHF/HU or DnaA) and it is positioned in the A + T-rich region adjacent to iterons. Experiments described here examine the effects of ATP, Mg2+ and temperature on the opening reaction. We show that the opening of the gamma origin can occur in the presence of ATP as well as AMP-PCP (a non-hydrolyzable ATP analog). This suggests that, for gamma origin, ATP hydrolysis may be unnecessary for open complex formation facilitated by His-pi.F107S. In the absence of ATP or Mg2+, His-pi.F107S yielded data suggestive of distortions in the iteron attributable to DNA bending rather than DNA melting. Our findings also demonstrate that ATP and pi stimulate open complex formation over a wide range of temperatures, but not at 0 degrees C. These and other results indicate that ATP and/or Mg2+ are not needed for His-pi.F107S binding to iterons and that ATP effects an allosteric change in the protein bound to gamma origin." @default.
• W2017150501 created "2016-06-24" @default.
• W2017150501 creator A5019304611 @default.
• W2017150501 creator A5030620890 @default.
• W2017150501 date "2003-10-15" @default.
• W2017150501 modified "2023-09-29" @default.
• W2017150501 title " Protein- and ATP-dependent transitions from 'closed' to 'open' complexes at the ori of plasmid R6K" @default.
• W2017150501 cites W124504601 @default.
• W2017150501 cites W1249064169 @default.
• W2017150501 cites W1423790558 @default.
• W2017150501 cites W1605567462 @default.
• W2017150501 cites W1963527323 @default.
• W2017150501 cites W1967024684 @default.
• W2017150501 cites W1970516061 @default.
• W2017150501 cites W1973763076 @default.
• W2017150501 cites W1976750364 @default.
• W2017150501 cites W1984217937 @default.
• W2017150501 cites W1984787245 @default.
• W2017150501 cites W1984830582 @default.
• W2017150501 cites W1989075626 @default.
• W2017150501 cites W1995466673 @default.
• W2017150501 cites W2006682691 @default.
• W2017150501 cites W2007253700 @default.
• W2017150501 cites W2008666178 @default.
• W2017150501 cites W2019114462 @default.
• W2017150501 cites W2025617204 @default.
• W2017150501 cites W2028080507 @default.
• W2017150501 cites W2030929667 @default.
• W2017150501 cites W2031012748 @default.
• W2017150501 cites W2032209646 @default.
• W2017150501 cites W2033576141 @default.
• W2017150501 cites W2040517347 @default.
• W2017150501 cites W2042517112 @default.
• W2017150501 cites W2046273708 @default.
• W2017150501 cites W2048347359 @default.
• W2017150501 cites W2049231478 @default.
• W2017150501 cites W2061135731 @default.
• W2017150501 cites W2064980063 @default.
• W2017150501 cites W2065328892 @default.
• W2017150501 cites W2068829724 @default.
• W2017150501 cites W2084874442 @default.
• W2017150501 cites W2087954717 @default.
• W2017150501 cites W2090623252 @default.
• W2017150501 cites W2137261337 @default.
• W2017150501 cites W2139316119 @default.
• W2017150501 cites W2151644073 @default.
• W2017150501 cites W2155835467 @default.
• W2017150501 cites W2166691943 @default.
• W2017150501 cites W4210962897 @default.
• W2017150501 cites W2042040255 @default.
• W2017150501 doi "https://doi.org/10.1093/nar/gkg809" @default.
• W2017150501 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/219486" @default.
• W2017150501 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/14530447" @default.
• W2017150501 hasPublicationYear "2003" @default.
• W2017150501 type Work @default.
• W2017150501 sameAs 2017150501 @default.
• W2017150501 citedByCount "5" @default.
• W2017150501 countsByYear W20171505012012 @default.
• W2017150501 countsByYear W20171505012013 @default.
• W2017150501 countsByYear W20171505012016 @default.
• W2017150501 countsByYear W20171505012023 @default.
• W2017150501 crossrefType "journal-article" @default.
• W2017150501 hasAuthorship W2017150501A5019304611 @default.
• W2017150501 hasAuthorship W2017150501A5030620890 @default.
• W2017150501 hasBestOaLocation W20171505011 @default.
• W2017150501 hasConcept C107827726 @default.
• W2017150501 hasConcept C12554922 @default.
• W2017150501 hasConcept C141315368 @default.
• W2017150501 hasConcept C153911025 @default.
• W2017150501 hasConcept C166342909 @default.
• W2017150501 hasConcept C181199279 @default.
• W2017150501 hasConcept C185592680 @default.
• W2017150501 hasConcept C22744801 @default.
• W2017150501 hasConcept C23265538 @default.
• W2017150501 hasConcept C53009064 @default.
• W2017150501 hasConcept C552990157 @default.
• W2017150501 hasConcept C55493867 @default.
• W2017150501 hasConcept C58337764 @default.
• W2017150501 hasConcept C71240020 @default.
• W2017150501 hasConcept C73573662 @default.
• W2017150501 hasConcept C8010536 @default.
• W2017150501 hasConcept C86803240 @default.
• W2017150501 hasConceptScore W2017150501C107827726 @default.
• W2017150501 hasConceptScore W2017150501C12554922 @default.
• W2017150501 hasConceptScore W2017150501C141315368 @default.
• W2017150501 hasConceptScore W2017150501C153911025 @default.
• W2017150501 hasConceptScore W2017150501C166342909 @default.
• W2017150501 hasConceptScore W2017150501C181199279 @default.
• W2017150501 hasConceptScore W2017150501C185592680 @default.
• W2017150501 hasConceptScore W2017150501C22744801 @default.
• W2017150501 hasConceptScore W2017150501C23265538 @default.
• W2017150501 hasConceptScore W2017150501C53009064 @default.
• W2017150501 hasConceptScore W2017150501C552990157 @default.
• W2017150501 hasConceptScore W2017150501C55493867 @default.
• W2017150501 hasConceptScore W2017150501C58337764 @default.
• W2017150501 hasConceptScore W2017150501C71240020 @default.
• W2017150501 hasConceptScore W2017150501C73573662 @default.
• W2017150501 hasConceptScore W2017150501C8010536 @default.

• next