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- W2017156924 abstract "The sensitivity of Western blots is limited by the avidity of antibody-antigen interactions, and by problems of specificity in interactions between antibodies and antigens from different species. Using rat and human keratins as the antigens, and a set of antibodies against human and rat keratins, this study demonstrates that mild treatment of nitrocellulose blots with trypsin and/or pepsin enhances the sensitivity of the assay and permits the cross-species demonstration of antigens that are not otherwise detectable." @default.
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- W2017156924 date "1991-07-01" @default.
- W2017156924 modified "2023-09-23" @default.
- W2017156924 title "Protease treatment of nitrocellulose-bound antigens enhances the sensitivity of Western blots" @default.
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- W2017156924 doi "https://doi.org/10.1016/0022-1759(91)90220-a" @default.
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