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• W2017177614 abstract "The transcription of poly [d(A-T)] by RNA polymerase from Escherichia coli in the presence of ATP and 4-thiouridine triphosphate (s4UTP) lead to the synthesis of poly[r(A-s4U)]. The investigation of the polymerisation kinetics furnished the kinetic parameter Km (ATP-s4UTP) = 0.33 mM and Vmax (ATP-s4UTP) = 204 nmoles AMP × mg enzyme−1× min−1. The structure of poly[r(A-s4U)] was proved by nearest neighbour analysis and calculation of the AMP/s4UMP ratio from the absorption spectrum of poly[r(A-s4U)]. As shown by sedimentation velocity analysis poly[r(A-s4U)] exhibited a mean s20, w value of 12 S. Enzymatic hydrolysis and thermal denaturation of poly[r(A-s4U)] revealed the existence of a large hyperchromicity in the absorption spectrum. The absorption-temperature profile of poly [r(A-s4U)] measured at 0.08 M Na+ showe a sharp cooperative transition with a Tm of 52°C." @default.
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• W2017177614 date "1971-04-01" @default.
• W2017177614 modified "2023-09-26" @default.
• W2017177614 title "The Synthesis of the Alternating Copolymer Poly [r(A-s4U)] by RNA Polymerase of Escherichia coli" @default.
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• W2017177614 doi "https://doi.org/10.1111/j.1432-1033.1971.tb01327.x" @default.
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