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• W2017180076 abstract "Abstract Waste plastics (e.g. polyethylene, polystyrene and polypropylene) have been converted to single‐cell protein using pyrolysis followed by fermentation. Conversion efficiencies of plastics to pyrolysate were 75–90% for polyethylene, 10–56% for polystyrene and 40–50% for polypropylene. Unpyrolysed residues were formed with polystyrene (6–14%) and polypropylene (14–30%), but not with polyethylene. Polyvinyl chloride produced hydrogen chloride fumes, together with 33–39% unpyrolysed residue, no fermentable pyrolysis products being formed. Polyethylene pyrolysate was fermented by the yeast, Candida tropicalis. Batch fermentation was carried out at 31°C and pH 5.5 on polyethylene pyrolysate in shaken flasks (100 cm 3 culture) and in an aerated fermenter (500 cm 3 culture). Maximum growth rate was 0.168 h −1 , cell yield was 0.47 ± 0.02 g g −1 pyrolysate used ( n = 3) and doubling time was 4–5 h, after 72 h growth on 1.0g pyrolysate 100 cm −3 culture. Continuous culture (dilution rate 0.10 h −1 ) gave a dry cell yield of 0.39 g g −1 pyrolysate utilised. Utilisation of pyrolysate was 49.0% in batch culture and 33.0% in continuous culture. The efficiency of conversion of polyethylene to biomass was 34–42% in batch culture. Emulsification and pristane‐solubilisation were studied as a means of dispersing waxy pyrolysates in culture media. Pristane did not support growth whereas the emulsifier [lecithin, Tween 85 and sodium glycocholate (1:2:2 by weight)] could support up to 27% of the growth observed on polyethylene pyrolysate. Although growth would occur without either emulsifier or pristane, use of pristane increased both dry cell yield (g g −1 pyrolysate used) and pyrolysate utilisation by 1.3 times in flask cultures. Crude protein content of cells cultured on polyethylene pyrolysate was 46.7 ± 2.4% ( n = 3) for batch culture cells, and 43.8 ± 0.3% ( n = 4) for continuous culture cells. The true protein content of Candida cells was about 17%. The protein had a favourable nutritional quality as judged by in‐vitro chemical tests." @default.
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• W2017180076 title "Conversion of waste plastics to single-cell protein by means of pyrolysis followed by fermentation" @default.
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• W2017180076 doi "https://doi.org/10.1002/jctb.503310109" @default.
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