FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2017182384> ?p ?o ?g. }

• W2017182384 endingPage "166" @default.
• W2017182384 startingPage "157" @default.
• W2017182384 abstract "Four experiments were conducted using mature Suffolk ewes to evaluate the effects of blood sample handling, processing and storage on measurements of the energy metabolites, β-hydroxybutyrate, total cholesterol, glucose, l-lactate, nonesterified fatty acid (NEFA), and triacylglycerol. In experiment 1 the effects of anticoagulants on metabolite measurements and packed-cell volume (PCV) were evaluated. Blood samples (n=12) were collected into one of four evacuated blood-collecting tubes: no anticoagulant (SER; yields serum), and plasma-yielding sodium heparin (HEP), sodium fluoride and potassium oxalate (NAF), and tripotassium ethylenediaminetetraacetic acid (K3EDTA). Anticoagulant affected (P<0.05) metabolite values, with total cholesterol, triacylglycerol, and l-lactate highest in SER, and glucose highest in HEP; NEFA and β-hydroxybutyrate measurements were not affected (P>0.10) by anticoagulant. In addition, among the plasma-yielding tubes, PCV was highest in HEP and lowest in NAF (P<0.01). Experiment 2 investigated the effects of repetitive freezing-thawing cycles of plasma on metabolite levels. Blood samples (n=20) were collected using NAF tubes, and plasma was stored at −20 °C and thawed in a water bath (21 °C) 0, 1, 2, and 3 times within 18 h after collection. Compared with fresh samples (Thaw 0), by thaw 2, l-lactate increased (P<0.01) 5%, and glucose and total cholesterol decreased (P<0.001) 13 and 4%, respectively. Plasma NEFA increased 10% (P<0.01) between thaws 0 and 1, but returned to fresh levels (Thaw 0) with subsequent freeze-thaw cycles (P<0.05). Plasma β-hydroxybutyrate decreased (P<0.01) between thaws 0 and 1, but there was no further decline with subsequent freeze-thaw cycles (P<0.05). Experiment 3 evaluated the effects of plasma storage temperature (−20 °C versus −80 °C) and length (0–180 days) on metabolite levels in blood samples (n=12) collected in NAF tubes. All plasma metabolites were affected by storage length (day effect, P<0.01) but only total cholesterol values were affected by storage temperature, with values being higher in plasma stored at −20 than −80 °C (P<0.01). Glucose measurements were higher in samples stored at −20 °C for up to 30 days, but were higher thereafter in samples stored at −80 °C (storage length × temperature effect, P<0.01). Experiment 4 evaluated the effects of anticoagulant (SER versus NAF) and whole blood storage (4 °C) for 0, 1, 3, and 7 days on metabolite concentrations. Glucose was higher (P<0.0001) in NAF, possibly the result of the presence of the glycolytic inhibitor, sodium fluoride, whereas l-lactate, NEFA, total cholesterol and β-hydroxybutyrate were higher in SER (P<0.01). Total cholesterol, l-lactate, and NEFA increased, whereas β-hydroxybutyrate decreased with days in storage (P<0.01). Development of hemolysis in the samples artifactually elevated glucose and NEFA values by as much as 41 and 230%, respectively. Attention to proper blood handling, processing, and storage procedures, and avoidance of hemolysis are important in blood clinical analyses and in the proper interpretation of experimental results." @default.
• W2017182384 created "2016-06-24" @default.
• W2017182384 creator A5004048235 @default.
• W2017182384 creator A5050468836 @default.
• W2017182384 creator A5073341333 @default.
• W2017182384 creator A5081616098 @default.
• W2017182384 creator A5082110362 @default.
• W2017182384 creator A5084485029 @default.
• W2017182384 date "2002-02-01" @default.
• W2017182384 modified "2023-10-01" @default.
• W2017182384 title "Effects of sample handling, processing, storage, and hemolysis on measurements of key energy metabolites in ovine blood" @default.
• W2017182384 cites W1895410483 @default.
• W2017182384 cites W1915930809 @default.
• W2017182384 cites W2006076149 @default.
• W2017182384 cites W2017513019 @default.
• W2017182384 cites W2034767525 @default.
• W2017182384 cites W2040304086 @default.
• W2017182384 cites W2076996897 @default.
• W2017182384 cites W2080637753 @default.
• W2017182384 cites W2122639895 @default.
• W2017182384 cites W2159103120 @default.
• W2017182384 cites W2184693321 @default.
• W2017182384 cites W2229420867 @default.
• W2017182384 cites W2316278432 @default.
• W2017182384 cites W2323701733 @default.
• W2017182384 cites W4297067344 @default.
• W2017182384 doi "https://doi.org/10.1016/s0921-4488(01)00266-8" @default.
• W2017182384 hasPublicationYear "2002" @default.
• W2017182384 type Work @default.
• W2017182384 sameAs 2017182384 @default.
• W2017182384 citedByCount "31" @default.
• W2017182384 countsByYear W20171823842013 @default.
• W2017182384 countsByYear W20171823842014 @default.
• W2017182384 countsByYear W20171823842017 @default.
• W2017182384 countsByYear W20171823842018 @default.
• W2017182384 countsByYear W20171823842019 @default.
• W2017182384 countsByYear W20171823842020 @default.
• W2017182384 countsByYear W20171823842021 @default.
• W2017182384 countsByYear W20171823842022 @default.
• W2017182384 countsByYear W20171823842023 @default.
• W2017182384 crossrefType "journal-article" @default.
• W2017182384 hasAuthorship W2017182384A5004048235 @default.
• W2017182384 hasAuthorship W2017182384A5050468836 @default.
• W2017182384 hasAuthorship W2017182384A5073341333 @default.
• W2017182384 hasAuthorship W2017182384A5081616098 @default.
• W2017182384 hasAuthorship W2017182384A5082110362 @default.
• W2017182384 hasAuthorship W2017182384A5084485029 @default.
• W2017182384 hasConcept C126322002 @default.
• W2017182384 hasConcept C140793950 @default.
• W2017182384 hasConcept C178790620 @default.
• W2017182384 hasConcept C185592680 @default.
• W2017182384 hasConcept C2777477808 @default.
• W2017182384 hasConcept C2779772378 @default.
• W2017182384 hasConcept C43617362 @default.
• W2017182384 hasConcept C537181965 @default.
• W2017182384 hasConcept C543025807 @default.
• W2017182384 hasConcept C55493867 @default.
• W2017182384 hasConcept C71924100 @default.
• W2017182384 hasConcept C86803240 @default.
• W2017182384 hasConceptScore W2017182384C126322002 @default.
• W2017182384 hasConceptScore W2017182384C140793950 @default.
• W2017182384 hasConceptScore W2017182384C178790620 @default.
• W2017182384 hasConceptScore W2017182384C185592680 @default.
• W2017182384 hasConceptScore W2017182384C2777477808 @default.
• W2017182384 hasConceptScore W2017182384C2779772378 @default.
• W2017182384 hasConceptScore W2017182384C43617362 @default.
• W2017182384 hasConceptScore W2017182384C537181965 @default.
• W2017182384 hasConceptScore W2017182384C543025807 @default.
• W2017182384 hasConceptScore W2017182384C55493867 @default.
• W2017182384 hasConceptScore W2017182384C71924100 @default.
• W2017182384 hasConceptScore W2017182384C86803240 @default.
• W2017182384 hasIssue "2" @default.
• W2017182384 hasLocation W20171823841 @default.
• W2017182384 hasOpenAccess W2017182384 @default.
• W2017182384 hasPrimaryLocation W20171823841 @default.
• W2017182384 hasRelatedWork W1546422309 @default.
• W2017182384 hasRelatedWork W1965051900 @default.
• W2017182384 hasRelatedWork W1972134878 @default.
• W2017182384 hasRelatedWork W1999101520 @default.
• W2017182384 hasRelatedWork W2114057458 @default.
• W2017182384 hasRelatedWork W2128137344 @default.
• W2017182384 hasRelatedWork W2135435480 @default.
• W2017182384 hasRelatedWork W2250113602 @default.
• W2017182384 hasRelatedWork W2331954937 @default.
• W2017182384 hasRelatedWork W3107008581 @default.
• W2017182384 hasVolume "43" @default.
• W2017182384 isParatext "false" @default.
• W2017182384 isRetracted "false" @default.
• W2017182384 magId "2017182384" @default.
• W2017182384 workType "article" @default.