FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2017189330> ?p ?o ?g. }

• W2017189330 endingPage "49" @default.
• W2017189330 startingPage "40" @default.
• W2017189330 abstract "Pili have only been discovered in the major Gram-positive pathogens in the past decade and they have been found to play an important role in colonisation and virulence. Pili have been shown to have many important functions including attachment to host tissues, mediating bacterial aggregation, biofilm formation and binding to proteins in the extracellular matrix. In this study, sortase-dependent pili have been found to be expressed on the surface of Finegoldia magna ALB8. F. magna is a Gram-positive anaerobic coccus that, primarily, is a commensal of the skin and mucous membranes, but has also been isolated from various clinical infection sites and is associated with soft-tissue abscesses, wound infections and bone and prosthetic joint infections. In this study, F. magna ALB8 was found to harbour three sortases at the pilus locus, two of which bear high similarity to class C sortases in Streptococcus pneumoniae. Two putative sortase-dependent pili proteins were found in the locus, with one being identified as the major pilus subunit, Fmp1 (F. magna pilus subunit 1), due to its high similarity to other major pilus proteins in prominent Gram-positive pathogens. The presence of sortase-dependent pili was confirmed experimentally through recombinant production of Fmp1 and production of antiserum. The Fmp1 antiserum was used in Western blot to show the presence of a high molecular weight protein ladder, characteristic of the presence of pili, in trypsin released cell wall surface proteins from F. magna. The presence of sortase-dependent pili was visually confirmed by transmission electron microscopy, which showed the binding of gold labelled anti-Fmp1 to individual pilus proteins along the pilus. Furthermore, pili could also be found to bind and interact with keratinocytes in the epidermal layer of human skin, suggesting an adhesive role for pili on F. magna. Our work represents the first description of pilus structures in F. magna. This discovery further elucidates F. magna physiology and allows for additional analysis of host-bacterial interactions in future studies." @default.
• W2017189330 created "2016-06-24" @default.
• W2017189330 creator A5000077565 @default.
• W2017189330 creator A5004049862 @default.
• W2017189330 creator A5030810354 @default.
• W2017189330 creator A5045374933 @default.
• W2017189330 creator A5049295334 @default.
• W2017189330 date "2014-06-01" @default.
• W2017189330 modified "2023-09-24" @default.
• W2017189330 title "Identification of pili on the surface of Finegoldia magna – A Gram-positive anaerobic cocci" @default.
• W2017189330 cites W1498894824 @default.
• W2017189330 cites W1532810787 @default.
• W2017189330 cites W1550209178 @default.
• W2017189330 cites W1561893393 @default.
• W2017189330 cites W1566240917 @default.
• W2017189330 cites W1607549514 @default.
• W2017189330 cites W1608238855 @default.
• W2017189330 cites W1929520690 @default.
• W2017189330 cites W1966994877 @default.
• W2017189330 cites W1967050835 @default.
• W2017189330 cites W1970532039 @default.
• W2017189330 cites W1973473578 @default.
• W2017189330 cites W1978191506 @default.
• W2017189330 cites W1988872938 @default.
• W2017189330 cites W1992162955 @default.
• W2017189330 cites W1993509275 @default.
• W2017189330 cites W1998045538 @default.
• W2017189330 cites W2002183209 @default.
• W2017189330 cites W2004548026 @default.
• W2017189330 cites W2009251244 @default.
• W2017189330 cites W2010283791 @default.
• W2017189330 cites W2011400095 @default.
• W2017189330 cites W2018893239 @default.
• W2017189330 cites W2024745748 @default.
• W2017189330 cites W2031249988 @default.
• W2017189330 cites W2037993016 @default.
• W2017189330 cites W2039817895 @default.
• W2017189330 cites W2041407246 @default.
• W2017189330 cites W2052397091 @default.
• W2017189330 cites W2055043387 @default.
• W2017189330 cites W2056577988 @default.
• W2017189330 cites W2065129970 @default.
• W2017189330 cites W2074060378 @default.
• W2017189330 cites W2097750123 @default.
• W2017189330 cites W2099580632 @default.
• W2017189330 cites W2103568524 @default.
• W2017189330 cites W2114339558 @default.
• W2017189330 cites W2127322768 @default.
• W2017189330 cites W2137136923 @default.
• W2017189330 cites W2138122846 @default.
• W2017189330 cites W2141444156 @default.
• W2017189330 cites W2141517634 @default.
• W2017189330 cites W2150488998 @default.
• W2017189330 cites W2152361995 @default.
• W2017189330 cites W2161025307 @default.
• W2017189330 cites W2169550436 @default.
• W2017189330 cites W2170345362 @default.
• W2017189330 cites W4232831728 @default.
• W2017189330 doi "https://doi.org/10.1016/j.anaerobe.2014.03.008" @default.
• W2017189330 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/24685556" @default.
• W2017189330 hasPublicationYear "2014" @default.
• W2017189330 type Work @default.
• W2017189330 sameAs 2017189330 @default.
• W2017189330 citedByCount "14" @default.
• W2017189330 countsByYear W20171893302016 @default.
• W2017189330 countsByYear W20171893302017 @default.
• W2017189330 countsByYear W20171893302018 @default.
• W2017189330 countsByYear W20171893302020 @default.
• W2017189330 countsByYear W20171893302021 @default.
• W2017189330 countsByYear W20171893302022 @default.
• W2017189330 crossrefType "journal-article" @default.
• W2017189330 hasAuthorship W2017189330A5000077565 @default.
• W2017189330 hasAuthorship W2017189330A5004049862 @default.
• W2017189330 hasAuthorship W2017189330A5030810354 @default.
• W2017189330 hasAuthorship W2017189330A5045374933 @default.
• W2017189330 hasAuthorship W2017189330A5049295334 @default.
• W2017189330 hasBestOaLocation W20171893301 @default.
• W2017189330 hasConcept C104292427 @default.
• W2017189330 hasConcept C104317684 @default.
• W2017189330 hasConcept C165175003 @default.
• W2017189330 hasConcept C174832134 @default.
• W2017189330 hasConcept C2776803919 @default.
• W2017189330 hasConcept C2779489039 @default.
• W2017189330 hasConcept C39103817 @default.
• W2017189330 hasConcept C523546767 @default.
• W2017189330 hasConcept C54355233 @default.
• W2017189330 hasConcept C55493867 @default.
• W2017189330 hasConcept C58123911 @default.
• W2017189330 hasConcept C60987743 @default.
• W2017189330 hasConcept C86803240 @default.
• W2017189330 hasConcept C89423630 @default.
• W2017189330 hasConceptScore W2017189330C104292427 @default.
• W2017189330 hasConceptScore W2017189330C104317684 @default.
• W2017189330 hasConceptScore W2017189330C165175003 @default.
• W2017189330 hasConceptScore W2017189330C174832134 @default.
• W2017189330 hasConceptScore W2017189330C2776803919 @default.
• W2017189330 hasConceptScore W2017189330C2779489039 @default.
• W2017189330 hasConceptScore W2017189330C39103817 @default.

• next