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- W2017190041 abstract "Alloreactive T cell populations can show skewing of T-cell antigen receptor (TCR) Vβ gene usage. The aims of the experiments were to compare in vivo and in vitro T cell alloresponses against donor alloantigens for TCR Vβ gene usage. T-cell cultures from renal biopsies taken during acute rejection and pretransplant mixed lymphocyte cultures (MLC) were established from five renal transplant patients. TCR Vβ gene usage, assessed with Vβ family specific antibodies, showed that up to five different Vβ families were significantly expanded. In four of five cases, there was close concordance between Vβ families expanded from the biopsy and in MLC. T-cell clones from one renal biopsy were specific for the mismatched donor alloantigen and showed similar TCR Vβ gene usage to the original T-cell line. The results show very similar patterns of TCR Vβ gene usage in alloreactive T cells generated ex vivo or in vitro." @default.
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- W2017190041 date "2005-12-27" @default.
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- W2017190041 title "Mixed Lymphocyte Cultures Can Predict TCR Vβ Repertoires of T Cells Infiltrating Kidney Transplants during Acute Rejection Episodes" @default.
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- W2017190041 doi "https://doi.org/10.1097/01.tp.0000181194.42642.97" @default.
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