Matches in SemOpenAlex for { <https://semopenalex.org/work/W2017190383> ?p ?o ?g. }
Showing items 1 to 90 of
90
with 100 items per page.
- W2017190383 endingPage "326" @default.
- W2017190383 startingPage "319" @default.
- W2017190383 abstract "Background: Russian thistle (Salsola kali) pollen is an important cause of pollinosis in areas where rainfall is not abundant. Our aim was to develop an ELISA for quantification of the major allergen of S. kali extracts, Sal k 1, and to assess the correlation of this allergen content with the allergenic activity of extracts. Methods: Sal k 1 was purified by ion exchange and gel permeation chromatography and identified by mass spectrometry. Monoclonal antibody 4C11 was used for capture at 5 µg/ml and biotin-labeled specific antiserum at 0.25 µg/ml served for detection. The allergenic activity of the pollen extracts was measured by enzyme allergosorbent test inhibition. Results: Sal k 1 reacted to 85% of sera from 40 S. kali-allergic patients and was able to inhibit 92% of the IgE-binding capacity of patients’ serum pool to the whole extract. The ELISA had a lineal range between 1.25 and 20 ng/ml of purified Sal k 1. The intra- and interassay coefficients of variation were lower than 5 and 10%, respectively. The assay was very sensitive since it had a detection limit of 0.08 ng/ml. No reactivity was found outside the Amaranthaceae family where only Kochia and Salicornia sp. gave significant reactivity. A good correlation (Spearman’s ρ = 0.92) was obtained between Sal k 1 content of different S. kali extracts and their IgE-binding activity. Conclusions: The results proved the usefulness of the two-site sandwich ELISA for aeroallergen control and for the standardization of S. kali pollen extracts intended for clinical use." @default.
- W2017190383 created "2016-06-24" @default.
- W2017190383 creator A5010849191 @default.
- W2017190383 creator A5028783112 @default.
- W2017190383 creator A5049768930 @default.
- W2017190383 creator A5054197125 @default.
- W2017190383 creator A5054917224 @default.
- W2017190383 creator A5055170906 @default.
- W2017190383 date "2010-01-01" @default.
- W2017190383 modified "2023-10-18" @default.
- W2017190383 title "The Russian Thistle(Salsola kali)Pollen Major Allergen, Sal k 1, Can Be Quantified in Allergenic Extracts and Airborne Pollen" @default.
- W2017190383 cites W1539224844 @default.
- W2017190383 cites W1653187305 @default.
- W2017190383 cites W1974976947 @default.
- W2017190383 cites W1979900325 @default.
- W2017190383 cites W1981637609 @default.
- W2017190383 cites W1982153352 @default.
- W2017190383 cites W1988716676 @default.
- W2017190383 cites W2001608628 @default.
- W2017190383 cites W2002956641 @default.
- W2017190383 cites W2009022904 @default.
- W2017190383 cites W2017425799 @default.
- W2017190383 cites W2026254290 @default.
- W2017190383 cites W2033232206 @default.
- W2017190383 cites W2075721128 @default.
- W2017190383 cites W2078594804 @default.
- W2017190383 cites W2082324016 @default.
- W2017190383 cites W2094530621 @default.
- W2017190383 cites W2100837269 @default.
- W2017190383 cites W2312829609 @default.
- W2017190383 cites W2413232299 @default.
- W2017190383 cites W2417793428 @default.
- W2017190383 cites W279163002 @default.
- W2017190383 cites W90366285 @default.
- W2017190383 cites W2108412156 @default.
- W2017190383 doi "https://doi.org/10.1159/000288285" @default.
- W2017190383 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/20185924" @default.
- W2017190383 hasPublicationYear "2010" @default.
- W2017190383 type Work @default.
- W2017190383 sameAs 2017190383 @default.
- W2017190383 citedByCount "4" @default.
- W2017190383 countsByYear W20171903832013 @default.
- W2017190383 countsByYear W20171903832014 @default.
- W2017190383 crossrefType "journal-article" @default.
- W2017190383 hasAuthorship W2017190383A5010849191 @default.
- W2017190383 hasAuthorship W2017190383A5028783112 @default.
- W2017190383 hasAuthorship W2017190383A5049768930 @default.
- W2017190383 hasAuthorship W2017190383A5054197125 @default.
- W2017190383 hasAuthorship W2017190383A5054917224 @default.
- W2017190383 hasAuthorship W2017190383A5055170906 @default.
- W2017190383 hasConcept C185592680 @default.
- W2017190383 hasConcept C203014093 @default.
- W2017190383 hasConcept C207480886 @default.
- W2017190383 hasConcept C2780510475 @default.
- W2017190383 hasConcept C2780590819 @default.
- W2017190383 hasConcept C2780618852 @default.
- W2017190383 hasConcept C43617362 @default.
- W2017190383 hasConcept C59822182 @default.
- W2017190383 hasConcept C86803240 @default.
- W2017190383 hasConceptScore W2017190383C185592680 @default.
- W2017190383 hasConceptScore W2017190383C203014093 @default.
- W2017190383 hasConceptScore W2017190383C207480886 @default.
- W2017190383 hasConceptScore W2017190383C2780510475 @default.
- W2017190383 hasConceptScore W2017190383C2780590819 @default.
- W2017190383 hasConceptScore W2017190383C2780618852 @default.
- W2017190383 hasConceptScore W2017190383C43617362 @default.
- W2017190383 hasConceptScore W2017190383C59822182 @default.
- W2017190383 hasConceptScore W2017190383C86803240 @default.
- W2017190383 hasIssue "4" @default.
- W2017190383 hasLocation W20171903831 @default.
- W2017190383 hasLocation W20171903832 @default.
- W2017190383 hasOpenAccess W2017190383 @default.
- W2017190383 hasPrimaryLocation W20171903831 @default.
- W2017190383 hasRelatedWork W1494139116 @default.
- W2017190383 hasRelatedWork W167150705 @default.
- W2017190383 hasRelatedWork W2011601592 @default.
- W2017190383 hasRelatedWork W2105684565 @default.
- W2017190383 hasRelatedWork W2364219807 @default.
- W2017190383 hasRelatedWork W2385775144 @default.
- W2017190383 hasRelatedWork W2777868487 @default.
- W2017190383 hasRelatedWork W3047778934 @default.
- W2017190383 hasRelatedWork W3201731073 @default.
- W2017190383 hasRelatedWork W90366285 @default.
- W2017190383 hasVolume "152" @default.
- W2017190383 isParatext "false" @default.
- W2017190383 isRetracted "false" @default.
- W2017190383 magId "2017190383" @default.
- W2017190383 workType "article" @default.