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- W2017191925 endingPage "157" @default.
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- W2017191925 abstract "A new ratiometric fluorescent probe derived from 4-hydroxy-N-butyl-1,8-naphthalimide (HNN) has been developed for selective detection of human carboxylesterase 2 (hCE2). The probe is designed by introducing benzoyl moiety to HNN, based on the intramolecular charge transfer (ICT) mechanism. The probe displays satisfying stability under physiological pH conditions with very low background fluorescence signal, but it can be rapidly hydrolyzed by hCE2 and release of HNN which leads to a remarkable red shift in emission spectra (148 nm). The newly designed probe exhibits excellent selectivity towards hCE2 over other human hydrolases, while the interference from various biologically relevant chemicals can be negligible. Its potential biological applications including inhibitor screening using human tissue preparations as enzyme sources, as well as fluorescence imaging of endogenous hCE2 in human living cells, have also been demonstrated." @default.
- W2017191925 created "2016-06-24" @default.
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- W2017191925 date "2014-12-01" @default.
- W2017191925 modified "2023-10-15" @default.
- W2017191925 title "A ratiometric fluorescent sensor for highly selective detection of human carboxylesterase 2 and its application in living cells" @default.
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- W2017191925 doi "https://doi.org/10.1016/j.snb.2014.08.066" @default.
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