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- W2017201930 abstract "Heterogeneous immunoassay (HI) has been recently used for on-chip biomolecule detection by many researchers due to its high specificity and precision. However, the protocols that have been published are too time-consuming. For HI based immunosensor to be attractive they should have short analysis times. In our work, a polydimethylsiloxane (PDMS) microfluidic device was fabricated to quantify a model analyte (bovine IgG) and used for optimizing the time required for each process. The results show that the incubation time of one single process could be reduced from typical 1 hour to 5 minutes without loss of performance. Our studies reveal that microfluidic devices are only effective in reducing incubation times for diffusion limited processes but not for interaction or adsorption limited process." @default.
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- W2017201930 date "2008-01-01" @default.
- W2017201930 modified "2023-09-26" @default.
- W2017201930 title "Time Optimization of Heterogeneous Immunoassay Using PDMS Microfluidic Chip for Quantitative Detection of a Model Analyte" @default.
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- W2017201930 doi "https://doi.org/10.1115/icnmm2008-62294" @default.
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