Matches in SemOpenAlex for { <https://semopenalex.org/work/W2017209345> ?p ?o ?g. }
Showing items 1 to 94 of
94
with 100 items per page.
- W2017209345 endingPage "3186" @default.
- W2017209345 startingPage "3178" @default.
- W2017209345 abstract "The nerve growth factor (NGF) receptor is a complex of two proteins, gp75 and the tyrosine kinase TrkA. Using fluorescence recovery after photobleaching, we have studied the diffusion properties of the TrkA receptor. For PC12 cells that express both gp75 and TrkA, TrkA was relatively immobile with only 28 ± 1% of receptor molecules free to diffuse with D = (3.64 ± 0.23) × 10-9 cm2/s. Addition of NGF decreased the mobile fraction to 21 ± 1% with D = (4.11 ± 0.18) × 10-9 cm2/s. Using the Sf9 baculovirus expression system, we were able to study TrkA in the absence and presence of gp75. On Sf9 cells, TrkA showed a mobile fraction of 46 ± 2% with D = (2.64 ± 0.21) × 10-9 cm2/s in the absence of gp75 and 43 ± 2% with D = (2.31 ± 0.25) × 10-9 cm2/s in its presence. Thus, gp75 did not alter TrkA mobility. Addition of NGF to the medium approximately halved the mobile fraction for TrkA in both the absence and presence of gp75. However, using a kinase-deficient mutant of TrkA, we found that ligand-induced immobilization requires an active kinase in the absence of gp75 but not in its presence. In addition, using point mutations at specific TrkA autophosphorylation sites, we determined that mobility is controlled by multiple phosphorylation sites, but the SHC binding site at Y490 may be particularly important for ligand-induced immobilization of TrkA. Therefore, two mechanisms lead to NGF-induced immobilization of TrkAthe first resulting from autophosphorylation of TrkA and the second occurring through TrkA's association with gp75." @default.
- W2017209345 created "2016-06-24" @default.
- W2017209345 creator A5011866001 @default.
- W2017209345 creator A5045886444 @default.
- W2017209345 creator A5056707687 @default.
- W2017209345 creator A5063851986 @default.
- W2017209345 creator A5071210752 @default.
- W2017209345 creator A5090059410 @default.
- W2017209345 date "1998-02-12" @default.
- W2017209345 modified "2023-10-18" @default.
- W2017209345 title "Mobility of TrkA Is Regulated by Phosphorylation and Interactions with the Low-Affinity NGF Receptor" @default.
- W2017209345 cites W143657778 @default.
- W2017209345 cites W1509043648 @default.
- W2017209345 cites W1565685840 @default.
- W2017209345 cites W1883249023 @default.
- W2017209345 cites W1896075940 @default.
- W2017209345 cites W1973863820 @default.
- W2017209345 cites W1987757645 @default.
- W2017209345 cites W1993356393 @default.
- W2017209345 cites W2002478108 @default.
- W2017209345 cites W2014313849 @default.
- W2017209345 cites W2017934591 @default.
- W2017209345 cites W2026233197 @default.
- W2017209345 cites W2027696867 @default.
- W2017209345 cites W2076606225 @default.
- W2017209345 cites W2081555648 @default.
- W2017209345 cites W2120594140 @default.
- W2017209345 cites W2127682895 @default.
- W2017209345 cites W2149335082 @default.
- W2017209345 cites W2154990535 @default.
- W2017209345 cites W74885494 @default.
- W2017209345 cites W97763042 @default.
- W2017209345 doi "https://doi.org/10.1021/bi9719253" @default.
- W2017209345 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/9485472" @default.
- W2017209345 hasPublicationYear "1998" @default.
- W2017209345 type Work @default.
- W2017209345 sameAs 2017209345 @default.
- W2017209345 citedByCount "13" @default.
- W2017209345 countsByYear W20172093452012 @default.
- W2017209345 crossrefType "journal-article" @default.
- W2017209345 hasAuthorship W2017209345A5011866001 @default.
- W2017209345 hasAuthorship W2017209345A5045886444 @default.
- W2017209345 hasAuthorship W2017209345A5056707687 @default.
- W2017209345 hasAuthorship W2017209345A5063851986 @default.
- W2017209345 hasAuthorship W2017209345A5071210752 @default.
- W2017209345 hasAuthorship W2017209345A5090059410 @default.
- W2017209345 hasConcept C11960822 @default.
- W2017209345 hasConcept C150109051 @default.
- W2017209345 hasConcept C170493617 @default.
- W2017209345 hasConcept C171034665 @default.
- W2017209345 hasConcept C185592680 @default.
- W2017209345 hasConcept C26659971 @default.
- W2017209345 hasConcept C2778423431 @default.
- W2017209345 hasConcept C41625074 @default.
- W2017209345 hasConcept C55493867 @default.
- W2017209345 hasConcept C56703729 @default.
- W2017209345 hasConcept C86803240 @default.
- W2017209345 hasConcept C95444343 @default.
- W2017209345 hasConcept C97029542 @default.
- W2017209345 hasConceptScore W2017209345C11960822 @default.
- W2017209345 hasConceptScore W2017209345C150109051 @default.
- W2017209345 hasConceptScore W2017209345C170493617 @default.
- W2017209345 hasConceptScore W2017209345C171034665 @default.
- W2017209345 hasConceptScore W2017209345C185592680 @default.
- W2017209345 hasConceptScore W2017209345C26659971 @default.
- W2017209345 hasConceptScore W2017209345C2778423431 @default.
- W2017209345 hasConceptScore W2017209345C41625074 @default.
- W2017209345 hasConceptScore W2017209345C55493867 @default.
- W2017209345 hasConceptScore W2017209345C56703729 @default.
- W2017209345 hasConceptScore W2017209345C86803240 @default.
- W2017209345 hasConceptScore W2017209345C95444343 @default.
- W2017209345 hasConceptScore W2017209345C97029542 @default.
- W2017209345 hasIssue "9" @default.
- W2017209345 hasLocation W20172093451 @default.
- W2017209345 hasLocation W20172093452 @default.
- W2017209345 hasOpenAccess W2017209345 @default.
- W2017209345 hasPrimaryLocation W20172093451 @default.
- W2017209345 hasRelatedWork W146630391 @default.
- W2017209345 hasRelatedWork W1973863820 @default.
- W2017209345 hasRelatedWork W2009479339 @default.
- W2017209345 hasRelatedWork W2017209345 @default.
- W2017209345 hasRelatedWork W2053000720 @default.
- W2017209345 hasRelatedWork W2071867041 @default.
- W2017209345 hasRelatedWork W2073167608 @default.
- W2017209345 hasRelatedWork W2075721364 @default.
- W2017209345 hasRelatedWork W2138688715 @default.
- W2017209345 hasRelatedWork W2141236543 @default.
- W2017209345 hasVolume "37" @default.
- W2017209345 isParatext "false" @default.
- W2017209345 isRetracted "false" @default.
- W2017209345 magId "2017209345" @default.
- W2017209345 workType "article" @default.