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- W2017218448 abstract "1.1. Reticulocytes of rabbits, sheep and humans but not those of rats or mice incorporate guanine into tRNA after the transcription of the polynucleotide chain is completed.2.2. The reaction is heat labile and is inhibited by iodoacetate, iodoacetamide and 8-azaguanine.3.3. No other purine is similarly incorporated into tRNA.4.4. The incorporated guanine is recovered as a single oligonucleotide after digestion of the guanylated tRNA with T1 ribonuclease and the incorporated guanine is present at the 3′ end of that oligonucleotide.5.5. The rate of guanylation in reticulocytes is concentration dependent within limits; and an apparent Km for guanylation of 2.2 · 10−5 M has been obtained. The Km for guanosine is 5.8 · 10−6 M.6.6. These findings point to an enzymatic mechanism for guanylation." @default.
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- W2017218448 date "1964-09-01" @default.
- W2017218448 modified "2023-10-18" @default.
- W2017218448 title "On the structure of neoguanosine" @default.
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- W2017218448 doi "https://doi.org/10.1016/0006-291x(64)90138-x" @default.
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