Matches in SemOpenAlex for { <https://semopenalex.org/work/W2017237260> ?p ?o ?g. }
- W2017237260 endingPage "1461" @default.
- W2017237260 startingPage "1454" @default.
- W2017237260 abstract "Different chromatographic separation modes have been combined, in off-line and on-line approaches, attempting to achieve enhanced separation power for the analysis of complex proteome samples. Among these, the use of RP-LC x RP-LC brings in the added advantages of high peak capacity in both dimensions, capability of full automation, and the likelihood of direct hyphenation to MS. In this contribution, we achieved enhanced resolution in the first dimension, by serially coupling Bio wide pore C18 columns at basic pH, at moderate pressure. Selectivity was tuned using one short (3 cm) C18 column packed with partially porous particles for fast second-dimension analysis, operated at acidic pH. The comprehensive system was configured around electronically activated two-position, ten-port valve for within-loop automated fraction collection/re-injection. A theoretical peak capacity of 3796 was calculated for the set-up employing one basic column in the first dimension, while a peak capacity of 10,686 was attained with four times the length of stationary phase, even at the expense of longer analysis time. The system showed very good reproducibility of retention times and chromatographic peak areas (RSD less than 1.2 and 4%, respectively)." @default.
- W2017237260 created "2016-06-24" @default.
- W2017237260 creator A5009040445 @default.
- W2017237260 creator A5009090739 @default.
- W2017237260 creator A5009688692 @default.
- W2017237260 creator A5070900474 @default.
- W2017237260 creator A5082948890 @default.
- W2017237260 date "2010-05-19" @default.
- W2017237260 modified "2023-10-14" @default.
- W2017237260 title "RP‐LC×RP‐LC analysis of a tryptic digest using a combination of totally porous and partially porous stationary phases" @default.
- W2017237260 cites W183402953 @default.
- W2017237260 cites W1964977456 @default.
- W2017237260 cites W1966635075 @default.
- W2017237260 cites W1969640920 @default.
- W2017237260 cites W1970696723 @default.
- W2017237260 cites W1972395654 @default.
- W2017237260 cites W1975398458 @default.
- W2017237260 cites W1975558168 @default.
- W2017237260 cites W1976358872 @default.
- W2017237260 cites W1976426449 @default.
- W2017237260 cites W1979026848 @default.
- W2017237260 cites W1979216053 @default.
- W2017237260 cites W1981593008 @default.
- W2017237260 cites W1988920835 @default.
- W2017237260 cites W1990203312 @default.
- W2017237260 cites W1992860360 @default.
- W2017237260 cites W1994280562 @default.
- W2017237260 cites W1995382473 @default.
- W2017237260 cites W1998415830 @default.
- W2017237260 cites W1999077518 @default.
- W2017237260 cites W2000430156 @default.
- W2017237260 cites W2004704197 @default.
- W2017237260 cites W2007286790 @default.
- W2017237260 cites W2009105593 @default.
- W2017237260 cites W2013755485 @default.
- W2017237260 cites W2016022626 @default.
- W2017237260 cites W2020520610 @default.
- W2017237260 cites W2021166225 @default.
- W2017237260 cites W2023096047 @default.
- W2017237260 cites W2023819040 @default.
- W2017237260 cites W2026465178 @default.
- W2017237260 cites W2028645231 @default.
- W2017237260 cites W2029100943 @default.
- W2017237260 cites W2034121267 @default.
- W2017237260 cites W2034356373 @default.
- W2017237260 cites W2036400336 @default.
- W2017237260 cites W2037837681 @default.
- W2017237260 cites W2038413380 @default.
- W2017237260 cites W2039171297 @default.
- W2017237260 cites W2041328507 @default.
- W2017237260 cites W2043050470 @default.
- W2017237260 cites W2043882273 @default.
- W2017237260 cites W2044970750 @default.
- W2017237260 cites W2046239792 @default.
- W2017237260 cites W2048333869 @default.
- W2017237260 cites W2050743934 @default.
- W2017237260 cites W2053971554 @default.
- W2017237260 cites W2054016284 @default.
- W2017237260 cites W2059870291 @default.
- W2017237260 cites W2062438096 @default.
- W2017237260 cites W2063859419 @default.
- W2017237260 cites W2064374182 @default.
- W2017237260 cites W2067303250 @default.
- W2017237260 cites W2070808569 @default.
- W2017237260 cites W2077314725 @default.
- W2017237260 cites W2077994693 @default.
- W2017237260 cites W2078662681 @default.
- W2017237260 cites W2079056345 @default.
- W2017237260 cites W2080883962 @default.
- W2017237260 cites W2082757239 @default.
- W2017237260 cites W2084173573 @default.
- W2017237260 cites W2085402253 @default.
- W2017237260 cites W2089813874 @default.
- W2017237260 cites W2090838755 @default.
- W2017237260 cites W2099839305 @default.
- W2017237260 cites W2110209421 @default.
- W2017237260 cites W2121716393 @default.
- W2017237260 cites W2153132589 @default.
- W2017237260 cites W90340583 @default.
- W2017237260 doi "https://doi.org/10.1002/jssc.200900816" @default.
- W2017237260 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/20209507" @default.
- W2017237260 hasPublicationYear "2010" @default.
- W2017237260 type Work @default.
- W2017237260 sameAs 2017237260 @default.
- W2017237260 citedByCount "35" @default.
- W2017237260 countsByYear W20172372602012 @default.
- W2017237260 countsByYear W20172372602013 @default.
- W2017237260 countsByYear W20172372602014 @default.
- W2017237260 countsByYear W20172372602015 @default.
- W2017237260 countsByYear W20172372602016 @default.
- W2017237260 countsByYear W20172372602017 @default.
- W2017237260 countsByYear W20172372602018 @default.
- W2017237260 countsByYear W20172372602019 @default.
- W2017237260 countsByYear W20172372602020 @default.
- W2017237260 countsByYear W20172372602021 @default.
- W2017237260 countsByYear W20172372602022 @default.
- W2017237260 crossrefType "journal-article" @default.
- W2017237260 hasAuthorship W2017237260A5009040445 @default.