Matches in SemOpenAlex for { <https://semopenalex.org/work/W2017242783> ?p ?o ?g. }
Showing items 1 to 75 of
75
with 100 items per page.
- W2017242783 endingPage "210" @default.
- W2017242783 startingPage "209" @default.
- W2017242783 abstract "Proteins are highly flexible molecules and often exhibit defined conformational changes in response to changes in the ambient temperature. Chicken egg white lysozyme has been previously shown to undergo an apparent structural change when warmed above the tetragonal/orthorhombic phase transition temperature. This is reflected by a change in the habit of the tetragonal and orthorhombic crystals so formed. In this study, we show that possible conformational changes induced by heating are stable and apparently non-reversible by simple cooling. Exposure of protein solutions to temperatures above the phase change transition temperature, before combining with precipitant solution to begin crystallization, reduces final crystal numbers. Protein that is briefly warmed to 37°C, then cooled shows no sign of reversal to the unheated nucleation behavior even after storage for 4 weeks at 4°C. The change in nucleation behavior of tetragonal lysozyme crystals, attributed to a structural shift, occurs faster the greater the exposure to temperature above the equi-solubility point for the two phases. Heating for 2 h at 48°C reduces crystal numbers by 20 fold in comparison to the same solution heated for the same time at 30°C. Thermal treatment of solutions is therefore a possible tool to reduce crystal numbers and increase crystal size. The effects of a protein's previous thermal history are now shown to be a potentially critical factor in subsequent macromolecule crystal nucleation and growth studies." @default.
- W2017242783 created "2016-06-24" @default.
- W2017242783 creator A5035484421 @default.
- W2017242783 creator A5046493480 @default.
- W2017242783 date "1978-05-01" @default.
- W2017242783 modified "2023-10-03" @default.
- W2017242783 title "Extension of the transition phenomenon in hen egg-white lysozyme crystals : the case of the monoclinic crystals" @default.
- W2017242783 cites W1970782282 @default.
- W2017242783 cites W1991603027 @default.
- W2017242783 cites W2048947819 @default.
- W2017242783 cites W2051540815 @default.
- W2017242783 cites W2063691025 @default.
- W2017242783 cites W2068333456 @default.
- W2017242783 cites W2315558541 @default.
- W2017242783 doi "https://doi.org/10.1016/s0300-9084(78)80757-3" @default.
- W2017242783 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/27243" @default.
- W2017242783 hasPublicationYear "1978" @default.
- W2017242783 type Work @default.
- W2017242783 sameAs 2017242783 @default.
- W2017242783 citedByCount "5" @default.
- W2017242783 crossrefType "journal-article" @default.
- W2017242783 hasAuthorship W2017242783A5035484421 @default.
- W2017242783 hasAuthorship W2017242783A5046493480 @default.
- W2017242783 hasConcept C104317684 @default.
- W2017242783 hasConcept C115624301 @default.
- W2017242783 hasConcept C185592680 @default.
- W2017242783 hasConcept C192562407 @default.
- W2017242783 hasConcept C194232998 @default.
- W2017242783 hasConcept C199360897 @default.
- W2017242783 hasConcept C2776966407 @default.
- W2017242783 hasConcept C2778029271 @default.
- W2017242783 hasConcept C2778484053 @default.
- W2017242783 hasConcept C31903555 @default.
- W2017242783 hasConcept C41008148 @default.
- W2017242783 hasConcept C55493867 @default.
- W2017242783 hasConcept C56273599 @default.
- W2017242783 hasConcept C61276311 @default.
- W2017242783 hasConcept C8010536 @default.
- W2017242783 hasConceptScore W2017242783C104317684 @default.
- W2017242783 hasConceptScore W2017242783C115624301 @default.
- W2017242783 hasConceptScore W2017242783C185592680 @default.
- W2017242783 hasConceptScore W2017242783C192562407 @default.
- W2017242783 hasConceptScore W2017242783C194232998 @default.
- W2017242783 hasConceptScore W2017242783C199360897 @default.
- W2017242783 hasConceptScore W2017242783C2776966407 @default.
- W2017242783 hasConceptScore W2017242783C2778029271 @default.
- W2017242783 hasConceptScore W2017242783C2778484053 @default.
- W2017242783 hasConceptScore W2017242783C31903555 @default.
- W2017242783 hasConceptScore W2017242783C41008148 @default.
- W2017242783 hasConceptScore W2017242783C55493867 @default.
- W2017242783 hasConceptScore W2017242783C56273599 @default.
- W2017242783 hasConceptScore W2017242783C61276311 @default.
- W2017242783 hasConceptScore W2017242783C8010536 @default.
- W2017242783 hasIssue "2" @default.
- W2017242783 hasLocation W20172427831 @default.
- W2017242783 hasLocation W20172427832 @default.
- W2017242783 hasOpenAccess W2017242783 @default.
- W2017242783 hasPrimaryLocation W20172427831 @default.
- W2017242783 hasRelatedWork W1850221914 @default.
- W2017242783 hasRelatedWork W2030772466 @default.
- W2017242783 hasRelatedWork W2061086122 @default.
- W2017242783 hasRelatedWork W2064636865 @default.
- W2017242783 hasRelatedWork W2095049086 @default.
- W2017242783 hasRelatedWork W2095538454 @default.
- W2017242783 hasRelatedWork W2126792960 @default.
- W2017242783 hasRelatedWork W3005583080 @default.
- W2017242783 hasRelatedWork W4234440170 @default.
- W2017242783 hasRelatedWork W68754254 @default.
- W2017242783 hasVolume "60" @default.
- W2017242783 isParatext "false" @default.
- W2017242783 isRetracted "false" @default.
- W2017242783 magId "2017242783" @default.
- W2017242783 workType "article" @default.