Matches in SemOpenAlex for { <https://semopenalex.org/work/W2017249468> ?p ?o ?g. }
Showing items 1 to 75 of
75
with 100 items per page.
- W2017249468 endingPage "429" @default.
- W2017249468 startingPage "418" @default.
- W2017249468 abstract "We previously reported on the generation of approximately 50-kb size defective genomes (DGs) which appeared to retain less than 2.2% of the standard Autographa californica nuclear polyhedrosis virus (AcMNPV) DNA between 85.0 and 87.2 MU while the rest of the virus DNA had been largely deleted (Lee and Krell, J. Virol., 66:4339-4347, 1992). To investigate these presumably repeated sequences further, we cloned and analyzed the most abundant hypermolar 1.80-kb Xhol DNA fragment as well as a minor but also supermolar 1.74-kb Xhol fragment of the DGs. These two DNA segments collectively covered 2371-bp of the standard AcMNPV DNA with a 1174-bp overlap around the Xhol site at 85.9 MU. Analysis of DGs by two-dimensional gel electrophoresis indicated that the 1.80- and 1.74-kb Xhol fragments (and most other novel Xhol fragments of the DG) were organized as tandem repeats in the DGs. We identified, in the DG population, small supercoiled DNA molecules approximately 1.0- to 8.2-kb (and possibly up to around 50 kb, the size of the major defective DNA species) in size and which contained the same DNA sequence as that of the major 1.80-kb repeat in the DGs. Furthermore, these cloned repeat sequences, represented by pLK1.80 and pLK1.74 showed AcMNPV infection-dependent autonomous replication, suggesting that an origin of DNA replication might reside within the HindIII to EcoRI segment (85.1 to 86.6 MU) of the HindIII-K fragment." @default.
- W2017249468 created "2016-06-24" @default.
- W2017249468 creator A5044748206 @default.
- W2017249468 creator A5068632932 @default.
- W2017249468 date "1994-07-01" @default.
- W2017249468 modified "2023-09-26" @default.
- W2017249468 title "Reiterated DNA Fragments in Defective Genomes of Autographa californica Nuclear Polyhedrosis Virus Are Competent for AcMNPV-Dependent DNA Replication" @default.
- W2017249468 doi "https://doi.org/10.1006/viro.1994.1358" @default.
- W2017249468 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/8009853" @default.
- W2017249468 hasPublicationYear "1994" @default.
- W2017249468 type Work @default.
- W2017249468 sameAs 2017249468 @default.
- W2017249468 citedByCount "52" @default.
- W2017249468 countsByYear W20172494682013 @default.
- W2017249468 countsByYear W20172494682014 @default.
- W2017249468 countsByYear W20172494682015 @default.
- W2017249468 countsByYear W20172494682017 @default.
- W2017249468 countsByYear W20172494682018 @default.
- W2017249468 countsByYear W20172494682020 @default.
- W2017249468 countsByYear W20172494682023 @default.
- W2017249468 crossrefType "journal-article" @default.
- W2017249468 hasAuthorship W2017249468A5044748206 @default.
- W2017249468 hasAuthorship W2017249468A5068632932 @default.
- W2017249468 hasConcept C104317684 @default.
- W2017249468 hasConcept C130123407 @default.
- W2017249468 hasConcept C141231307 @default.
- W2017249468 hasConcept C153911025 @default.
- W2017249468 hasConcept C159047783 @default.
- W2017249468 hasConcept C22744801 @default.
- W2017249468 hasConcept C2522874641 @default.
- W2017249468 hasConcept C2777773057 @default.
- W2017249468 hasConcept C2779665282 @default.
- W2017249468 hasConcept C2780199244 @default.
- W2017249468 hasConcept C40767141 @default.
- W2017249468 hasConcept C54355233 @default.
- W2017249468 hasConcept C552990157 @default.
- W2017249468 hasConcept C73573662 @default.
- W2017249468 hasConcept C86803240 @default.
- W2017249468 hasConceptScore W2017249468C104317684 @default.
- W2017249468 hasConceptScore W2017249468C130123407 @default.
- W2017249468 hasConceptScore W2017249468C141231307 @default.
- W2017249468 hasConceptScore W2017249468C153911025 @default.
- W2017249468 hasConceptScore W2017249468C159047783 @default.
- W2017249468 hasConceptScore W2017249468C22744801 @default.
- W2017249468 hasConceptScore W2017249468C2522874641 @default.
- W2017249468 hasConceptScore W2017249468C2777773057 @default.
- W2017249468 hasConceptScore W2017249468C2779665282 @default.
- W2017249468 hasConceptScore W2017249468C2780199244 @default.
- W2017249468 hasConceptScore W2017249468C40767141 @default.
- W2017249468 hasConceptScore W2017249468C54355233 @default.
- W2017249468 hasConceptScore W2017249468C552990157 @default.
- W2017249468 hasConceptScore W2017249468C73573662 @default.
- W2017249468 hasConceptScore W2017249468C86803240 @default.
- W2017249468 hasIssue "1" @default.
- W2017249468 hasLocation W20172494681 @default.
- W2017249468 hasLocation W20172494682 @default.
- W2017249468 hasOpenAccess W2017249468 @default.
- W2017249468 hasPrimaryLocation W20172494681 @default.
- W2017249468 hasRelatedWork W1518040381 @default.
- W2017249468 hasRelatedWork W1557634167 @default.
- W2017249468 hasRelatedWork W1776624303 @default.
- W2017249468 hasRelatedWork W1973341961 @default.
- W2017249468 hasRelatedWork W1975169637 @default.
- W2017249468 hasRelatedWork W2071218254 @default.
- W2017249468 hasRelatedWork W2079086650 @default.
- W2017249468 hasRelatedWork W2143428728 @default.
- W2017249468 hasRelatedWork W2188183750 @default.
- W2017249468 hasRelatedWork W2215500351 @default.
- W2017249468 hasVolume "202" @default.
- W2017249468 isParatext "false" @default.
- W2017249468 isRetracted "false" @default.
- W2017249468 magId "2017249468" @default.
- W2017249468 workType "article" @default.