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• W2017252124 abstract "Single-stranded M13mp10 DNA containing a 144-bp mutational target sequence in the lacZ alpha gene was treated with singlet oxygen (1O2) generated by thermodissociation of the endoperoxide of 3,3'-(1,4-naphthalene-1,4-diyl)dipropionate (NDPO2). After transfection to non-SOS-induced E. coli cells, 32 mutants preselected for a mutation in the 144-bp target were collected and analyzed by DNA sequencing. One-G deletions represented the predominant type of mutation accounting for 50% of the mutations analyzed. The remaining part appeared to consist of base substitutions, i.e. G-->T transversions (34%), C-->T transitions (12.5%) and one T-->C transition (3%). Sixty percent of the mutations were found in two major mutational hotspots. We conclude that the predominant one-G deletions are due to a guanine reaction product which might be specific for 1O2." @default.
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• W2017252124 date "1994-08-01" @default.
• W2017252124 modified "2023-10-17" @default.
• W2017252124 title "The formation of one-G deletions as a consequence of singlet-oxygen-induced DNA dmage" @default.
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• W2017252124 doi "https://doi.org/10.1016/0027-5107(94)90041-8" @default.
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