FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2017256112> ?p ?o ?g. }

• W2017256112 endingPage "293" @default.
• W2017256112 startingPage "285" @default.
• W2017256112 abstract "The microenvironment within solid tumors has often been shown to exhibit an acidic extracellular pH. Although the morphologic and functional differences in natural killer (NK) cells of the liver and spleen have been reported previously under physiological conditions, the difference under acidic conditions is still unclear. This study was to investigate the differences in the morphological and functional characteristics between rat liver and spleen NK cells under normal and acidic conditions in vitro. Liver and spleen NK cells were isolated and purified from Sprague-Dawley rats by density gradient centrifugation and the Dynabeads® FlowComp™ Flexi system, and stimulated for 4 days with or without IL-2 or treated with low pH or control for different times. Morphology was examined by scanning electron microscopy (SEM) and transmission electron microscopy (TEM), cell death and proliferation assays were performed by flow cytometry, IFN-γ production was tested by ELISA, and cytotoxic activity was evaluated by lactate dehydrogenase (LDH) release assay. Liver NK cells had significantly higher levels of cytotoxic activity than spleen NK cells under normal and acidic conditions, and the maximum difference was observed at pH 5.6. Further analysis revealed that the cytotoxic activity of NK cells was correlated with morphology, cell death, proliferative activity and IFN-γ production. By TEM, liver NK cells contained a greater number of electron-dense granules per cell at pH 5.6. Moreover, a modest elevation of cell death and reduction of proliferation of liver NK cells occurred within a range of 5.6–7.2. Interestingly, an acidic extracellular pH only marginally, and not significantly, suppressed IFN-γ production by liver NK cells. The sharp morphological and functional differences shown by the two types of NK cells in vitro indicate that liver NK cells are unexpectedly resistant to pH shock." @default.
• W2017256112 created "2016-06-24" @default.
• W2017256112 creator A5004295485 @default.
• W2017256112 creator A5017409095 @default.
• W2017256112 creator A5033290671 @default.
• W2017256112 creator A5053519636 @default.
• W2017256112 creator A5076636647 @default.
• W2017256112 creator A5076699095 @default.
• W2017256112 creator A5078200906 @default.
• W2017256112 creator A5088941984 @default.
• W2017256112 date "2012-06-01" @default.
• W2017256112 modified "2023-10-18" @default.
• W2017256112 title "Functional distinction of rat liver natural killer cells from spleen natural killer cells under normal and acidic conditions in vitro" @default.
• W2017256112 cites W1556867369 @default.
• W2017256112 cites W1565702878 @default.
• W2017256112 cites W1970753581 @default.
• W2017256112 cites W1987311790 @default.
• W2017256112 cites W1988147130 @default.
• W2017256112 cites W1990078051 @default.
• W2017256112 cites W1999334756 @default.
• W2017256112 cites W1999381809 @default.
• W2017256112 cites W2000003423 @default.
• W2017256112 cites W2021287775 @default.
• W2017256112 cites W2037555606 @default.
• W2017256112 cites W2046926103 @default.
• W2017256112 cites W2047181165 @default.
• W2017256112 cites W2050051573 @default.
• W2017256112 cites W2052086040 @default.
• W2017256112 cites W2071742694 @default.
• W2017256112 cites W2081536518 @default.
• W2017256112 cites W2133709322 @default.
• W2017256112 cites W2138095749 @default.
• W2017256112 cites W2165884308 @default.
• W2017256112 cites W2314331148 @default.
• W2017256112 doi "https://doi.org/10.1016/s1499-3872(12)60162-3" @default.
• W2017256112 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/22672823" @default.
• W2017256112 hasPublicationYear "2012" @default.
• W2017256112 type Work @default.
• W2017256112 sameAs 2017256112 @default.
• W2017256112 citedByCount "13" @default.
• W2017256112 countsByYear W20172561122013 @default.
• W2017256112 countsByYear W20172561122014 @default.
• W2017256112 countsByYear W20172561122015 @default.
• W2017256112 countsByYear W20172561122018 @default.
• W2017256112 countsByYear W20172561122019 @default.
• W2017256112 countsByYear W20172561122020 @default.
• W2017256112 countsByYear W20172561122021 @default.
• W2017256112 crossrefType "journal-article" @default.
• W2017256112 hasAuthorship W2017256112A5004295485 @default.
• W2017256112 hasAuthorship W2017256112A5017409095 @default.
• W2017256112 hasAuthorship W2017256112A5033290671 @default.
• W2017256112 hasAuthorship W2017256112A5053519636 @default.
• W2017256112 hasAuthorship W2017256112A5076636647 @default.
• W2017256112 hasAuthorship W2017256112A5076699095 @default.
• W2017256112 hasAuthorship W2017256112A5078200906 @default.
• W2017256112 hasAuthorship W2017256112A5088941984 @default.
• W2017256112 hasConcept C153911025 @default.
• W2017256112 hasConcept C154317977 @default.
• W2017256112 hasConcept C181199279 @default.
• W2017256112 hasConcept C185592680 @default.
• W2017256112 hasConcept C190283241 @default.
• W2017256112 hasConcept C202751555 @default.
• W2017256112 hasConcept C203014093 @default.
• W2017256112 hasConcept C2777318727 @default.
• W2017256112 hasConcept C2778102761 @default.
• W2017256112 hasConcept C2780931953 @default.
• W2017256112 hasConcept C28406088 @default.
• W2017256112 hasConcept C553184892 @default.
• W2017256112 hasConcept C55493867 @default.
• W2017256112 hasConcept C86803240 @default.
• W2017256112 hasConceptScore W2017256112C153911025 @default.
• W2017256112 hasConceptScore W2017256112C154317977 @default.
• W2017256112 hasConceptScore W2017256112C181199279 @default.
• W2017256112 hasConceptScore W2017256112C185592680 @default.
• W2017256112 hasConceptScore W2017256112C190283241 @default.
• W2017256112 hasConceptScore W2017256112C202751555 @default.
• W2017256112 hasConceptScore W2017256112C203014093 @default.
• W2017256112 hasConceptScore W2017256112C2777318727 @default.
• W2017256112 hasConceptScore W2017256112C2778102761 @default.
• W2017256112 hasConceptScore W2017256112C2780931953 @default.
• W2017256112 hasConceptScore W2017256112C28406088 @default.
• W2017256112 hasConceptScore W2017256112C553184892 @default.
• W2017256112 hasConceptScore W2017256112C55493867 @default.
• W2017256112 hasConceptScore W2017256112C86803240 @default.
• W2017256112 hasIssue "3" @default.
• W2017256112 hasLocation W20172561121 @default.
• W2017256112 hasLocation W20172561122 @default.
• W2017256112 hasOpenAccess W2017256112 @default.
• W2017256112 hasPrimaryLocation W20172561121 @default.
• W2017256112 hasRelatedWork W2041039743 @default.
• W2017256112 hasRelatedWork W2152593596 @default.
• W2017256112 hasRelatedWork W2372494870 @default.
• W2017256112 hasRelatedWork W2373412498 @default.
• W2017256112 hasRelatedWork W2374966914 @default.
• W2017256112 hasRelatedWork W2382426014 @default.
• W2017256112 hasRelatedWork W2403432132 @default.
• W2017256112 hasRelatedWork W2565726034 @default.
• W2017256112 hasRelatedWork W2917719117 @default.

• next