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- W2017256264 abstract "Abstract The distribution of radioactivity in the cytoplasm, spore coats, and exudates during germination of Bacillus licheniformis spores labeled with radioactive l-alanine and l-valine was determined. When the radioactive spores were germinated with nonradioactive l-alanine and l-valine, radioactivity was released after germination medium, with very little radioactivity released after germination was complete. Most of the radioactivity had its origin in the cytoplasm in l-alanine-induced germination, but with l-valine approximately 50% of the radioactivity was released from the spore coats. The radioactivity released was associated with ninhydrin-positive components on paper chromatograms, one at the point of application to the paper, the other at the Rf value of alanine. When l-alanine was used to germinate the spores, the greater portion of radioactivity (66%) was in the alanine spot. When l-valine was the germinant, approximately 50% of the radioactivity in the exudates remained as a nonmohile ninhydrin-positive component. When radioactive spores were germinated with l-alanine, free d-alanine was released into the germination medium, with the concentration increasing throughout a 4-hr incubation period. d-alanine was not released when l-valine was used as the spore germinant. Spores germinated with either l-alanine or l-valine also released dipicolinic acid (DPA) into the germination medium after germination. The release of DPA was somewhat slower than that of d-alanine, with d-alanine appearing in the germination exudates during the process of germination, and DPA being released only after germination was practically complete." @default.
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- W2017256264 date "1965-09-01" @default.
- W2017256264 modified "2023-09-26" @default.
- W2017256264 title "Role of Amino Acids in Germination of Bacillus licheniformis Spores. II. Effect on Radioactive Spores" @default.
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- W2017256264 doi "https://doi.org/10.3168/jds.s0022-0302(65)88424-7" @default.
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