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- W2017262124 abstract "A radioimmunoassay (RIA) for the measurement of antibody to hepatitis B surface antigen (anti-HBs) in human and non-human sera is described. The principle is based upon our earlier observation that anti-HBs and hepatitis B surface antigen (HBsAg) are soluble in 4.5% w/v polyethylene glycol 4000 but that anti-HBsHBsAg complex precipitated. The use of activated charcoal suspended in polyethylene glycol and dextran solution facilitated the separation of this complex by centrifugation, thus improving reproducibility and sensitivity. The test is approx. 3–5000 times as sensitive as counterelectrophoresis. In a survey of a normal blood donor population, with an incidence of approx. 0.1% positive for HBsAg by RIA, 7–8% were found to have anti-HBs by this method." @default.
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- W2017262124 date "1975-06-01" @default.
- W2017262124 modified "2023-09-27" @default.
- W2017262124 title "Detection of hepatitis B antibody by a single-antibody radioimmunoassay" @default.
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- W2017262124 doi "https://doi.org/10.1016/0022-1759(75)90014-9" @default.
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