FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2017267989> ?p ?o ?g. }

• W2017267989 endingPage "874" @default.
• W2017267989 startingPage "867" @default.
• W2017267989 abstract "The Thermus thermophilus hypothetical protein TTHA1280 belongs to a family of predicted S-adenosyl-l-methionine (AdoMet) dependent RNA methyltransferases (MTases) present in many bacterial and archaeal species. Inspection of amino-acid sequence motifs common to class I Rossmann-fold-like MTases suggested a specific role as an RNA 5-methyluridine MTase. Selenomethionine (SeMet) labelled and native versions of the protein were expressed, purified and crystallized. Two crystal forms of the SeMet-labelled apoprotein were obtained: SeMet-ApoI and SeMet-ApoII. Cocrystallization of the native protein with S-­adenosyl-l-homocysteine (AdoHcy) yielded a third crystal form, Native-AdoHcy. The SeMet-ApoI structure was solved by the multiple anomalous dispersion method and refined at 2.55 Å resolution. The SeMet-ApoII and Native-AdoHcy structures were solved by molecular replacement and refined at 1.80 and 2.60 Å, respectively. TTHA1280 formed a homodimer in the crystals and in solution. Each subunit folds into a three-domain structure composed of a small N-terminal PUA domain, a central α/β-domain and a C-terminal Rossmann-fold-like MTase domain. The three domains form an overall clamp-like shape, with the putative active site facing a deep cleft. The architecture of the active site is consistent with specific recognition of uridine and catalysis of methyl transfer to the 5-carbon position. The cleft is suitable in size and charge distribution for binding single-stranded RNA." @default.
• W2017267989 created "2016-06-24" @default.
• W2017267989 creator A5000593478 @default.
• W2017267989 creator A5012045946 @default.
• W2017267989 creator A5025116997 @default.
• W2017267989 creator A5027781657 @default.
• W2017267989 creator A5053618550 @default.
• W2017267989 creator A5075017610 @default.
• W2017267989 creator A5077817293 @default.
• W2017267989 date "2005-09-30" @default.
• W2017267989 modified "2023-10-07" @default.
• W2017267989 title "Structures of a putative RNA 5-methyluridine methyltransferase,<i>Thermus thermophilus</i>TTHA1280, and its complex with<i>S</i>-adenosyl-<scp>L</scp>-homocysteine" @default.
• W2017267989 cites W1539796472 @default.
• W2017267989 cites W1598610569 @default.
• W2017267989 cites W1965277349 @default.
• W2017267989 cites W1965410991 @default.
• W2017267989 cites W1978683810 @default.
• W2017267989 cites W1984451904 @default.
• W2017267989 cites W1985629904 @default.
• W2017267989 cites W1986724278 @default.
• W2017267989 cites W1989604770 @default.
• W2017267989 cites W1995017064 @default.
• W2017267989 cites W1998564583 @default.
• W2017267989 cites W2013083986 @default.
• W2017267989 cites W2022058405 @default.
• W2017267989 cites W2029582401 @default.
• W2017267989 cites W2032407233 @default.
• W2017267989 cites W2056370411 @default.
• W2017267989 cites W2100071542 @default.
• W2017267989 cites W2106882534 @default.
• W2017267989 cites W2117954450 @default.
• W2017267989 cites W2136495792 @default.
• W2017267989 cites W2142126483 @default.
• W2017267989 cites W2143694829 @default.
• W2017267989 cites W2155060633 @default.
• W2017267989 doi "https://doi.org/10.1107/s1744309105029842" @default.
• W2017267989 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/1991318" @default.
• W2017267989 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/16511182" @default.
• W2017267989 hasPublicationYear "2005" @default.
• W2017267989 type Work @default.
• W2017267989 sameAs 2017267989 @default.
• W2017267989 citedByCount "9" @default.
• W2017267989 countsByYear W20172679892012 @default.
• W2017267989 countsByYear W20172679892013 @default.
• W2017267989 countsByYear W20172679892014 @default.
• W2017267989 countsByYear W20172679892017 @default.
• W2017267989 crossrefType "journal-article" @default.
• W2017267989 hasAuthorship W2017267989A5000593478 @default.
• W2017267989 hasAuthorship W2017267989A5012045946 @default.
• W2017267989 hasAuthorship W2017267989A5025116997 @default.
• W2017267989 hasAuthorship W2017267989A5027781657 @default.
• W2017267989 hasAuthorship W2017267989A5053618550 @default.
• W2017267989 hasAuthorship W2017267989A5075017610 @default.
• W2017267989 hasAuthorship W2017267989A5077817293 @default.
• W2017267989 hasBestOaLocation W20172679892 @default.
• W2017267989 hasConcept C104292427 @default.
• W2017267989 hasConcept C104317684 @default.
• W2017267989 hasConcept C181199279 @default.
• W2017267989 hasConcept C185592680 @default.
• W2017267989 hasConcept C2776376580 @default.
• W2017267989 hasConcept C2780883830 @default.
• W2017267989 hasConcept C2781259782 @default.
• W2017267989 hasConcept C33288867 @default.
• W2017267989 hasConcept C547475151 @default.
• W2017267989 hasConcept C552990157 @default.
• W2017267989 hasConcept C55493867 @default.
• W2017267989 hasConcept C67705224 @default.
• W2017267989 hasConcept C71240020 @default.
• W2017267989 hasConcept C8010536 @default.
• W2017267989 hasConcept C86803240 @default.
• W2017267989 hasConcept C91965660 @default.
• W2017267989 hasConceptScore W2017267989C104292427 @default.
• W2017267989 hasConceptScore W2017267989C104317684 @default.
• W2017267989 hasConceptScore W2017267989C181199279 @default.
• W2017267989 hasConceptScore W2017267989C185592680 @default.
• W2017267989 hasConceptScore W2017267989C2776376580 @default.
• W2017267989 hasConceptScore W2017267989C2780883830 @default.
• W2017267989 hasConceptScore W2017267989C2781259782 @default.
• W2017267989 hasConceptScore W2017267989C33288867 @default.
• W2017267989 hasConceptScore W2017267989C547475151 @default.
• W2017267989 hasConceptScore W2017267989C552990157 @default.
• W2017267989 hasConceptScore W2017267989C55493867 @default.
• W2017267989 hasConceptScore W2017267989C67705224 @default.
• W2017267989 hasConceptScore W2017267989C71240020 @default.
• W2017267989 hasConceptScore W2017267989C8010536 @default.
• W2017267989 hasConceptScore W2017267989C86803240 @default.
• W2017267989 hasConceptScore W2017267989C91965660 @default.
• W2017267989 hasIssue "10" @default.
• W2017267989 hasLocation W20172679891 @default.
• W2017267989 hasLocation W20172679892 @default.
• W2017267989 hasLocation W20172679893 @default.
• W2017267989 hasLocation W20172679894 @default.
• W2017267989 hasOpenAccess W2017267989 @default.
• W2017267989 hasPrimaryLocation W20172679891 @default.
• W2017267989 hasRelatedWork W2003083382 @default.
• W2017267989 hasRelatedWork W2054210259 @default.
• W2017267989 hasRelatedWork W2089104969 @default.
• W2017267989 hasRelatedWork W2475490100 @default.

• next