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- W2017281154 abstract "In the present study, we determined whether α-7 subunit containing nicotinic acetylcholine receptors (nAChRs) are expressed by neurons within the pre-Botzinger complex (pre-BotC), bulbospinal, and phrenic motor nuclei in the rat. α-7 Immunohistochemistry combined with cholera toxin B (CTB), a retrograde tracer was used to detect expression of α-7 nAChRs by phrenic motor and bulbospinal neurons. Neurokinin-1 receptor immunoreactivity was used as a marker for pre-BotC neurons. Of the CTB-positive neurons in the phrenic nuclei, 60% exhibited immunoreactivity for α-7 nAChRs. Of the bulbospinal neurons in the paramedian reticular nuclei (PMn), gigantocellular nuclei (Gi), raphe nuclei, rostral ventrolateral medulla (RVLM) and nucleus tractus solitarius, 20–50% were found to express α-7 nAChR immunoreactivity. Of the peudorabies virus (PRV) labeled bulbospinal neurons in PMn, Gi, raphe and RVLM, 9–12% co-expressed α-7 nAChRs. Immunoreactivity for α-7 nAChRs was also detected in 57% of the neurokinin-1 receptor containing neurons presumed to reside in pre-BotC. These findings suggest that nicotinic cholinergic regulation of the chest wall pumping muscles may occur at multiple levels of the central nervous system." @default.
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- W2017281154 date "2004-07-01" @default.
- W2017281154 modified "2023-10-17" @default.
- W2017281154 title "Expression of α-7 nAChRs on spinal cord–brainstem neurons controlling inspiratory drive to the diaphragm" @default.
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- W2017281154 doi "https://doi.org/10.1016/j.resp.2004.03.006" @default.
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