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• W2017281251 abstract "Complete retinal regeneration occurs after the removal of the whole tissue in mature Xenopus laevis, as well as in the newt. Here, we produced F1 and F2 lines of transgenic X. laevis containing an EGFP gene under a translation elongation factor 1-α (ef1-α) promoter and investigated how the gene is reactivated in retinal pigmented epithelial (RPE) cells when the neural retina (NR) is removed. The results showed that EGFP expression is reduced in the adult ocular tissues of nonmanipulated transgenic animals, and EGFP-expressing cells are occasionally found heterogeneously in the lens, NR and RPE tissues. During retinal regeneration, the EGFP gene is reactivated in the RPE and ciliary marginal cells. Transgenic animals were also used for a transplant study because of the genetic marker of the donor tissue. Transplanted RPE clearly transdifferentiated to regenerate the retina in the ocular chamber. This study is, to our knowledge, the first report of a transgenic study of amphibian retinal regeneration, and the approach is promising for future molecular analyses. genesis 50:642–650, 2012. © 2012 Wiley Periodicals, Inc." @default.
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• W2017281251 date "2012-04-03" @default.
• W2017281251 modified "2023-09-25" @default.
• W2017281251 title "Transgenic Xenopus laevis with the ef1-α promoter as an experimental tool for amphibian retinal regeneration study" @default.
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• W2017281251 doi "https://doi.org/10.1002/dvg.22024" @default.
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