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- W2017287332 abstract "We describe a method, complementary strand analysis (CSA), for separating alleles potentially from any heterozygous genetic locus. Locus specific PCR is performed generating two allelic products. The antisense strands are isolated and hybridised with a sense reference strand to form a chimeric DNA duplex for each allele which is then separated by non-denaturing PAGE. We demonstrate the application of CSA for separation of highly polymorphic HLA-A, -B and -Cw alleles and characterisation of HLA identity in related bone marrow donors and patients. CSA is capable of resolving one nucleotide differences in a DNA fragment nearly as large as a kilobase in length." @default.
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- W2017287332 date "1997-06-01" @default.
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- W2017287332 title "Complementary strand analysis: a new approach for allelic separation in complex polyallelic genetic systems" @default.
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- W2017287332 doi "https://doi.org/10.1093/nar/25.11.2236" @default.
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