FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2017289279> ?p ?o ?g. }

• W2017289279 endingPage "48" @default.
• W2017289279 startingPage "39" @default.
• W2017289279 abstract "Chronic, low-level exposure to arsenic frequently results in skin, lung, bladder, and kidney cancer. Since arsenic is primarily excreted via the kidney, this study focused on this target tissue. Gene array was used as a sensitive low-level monitor of the impact of arsenic on this target tissue. Arsenite [As(III)] was chosen as the chemical species of arsenic since As(III) species are touted as the cellular toxic form of arsenic. Human embryonic kidney cell line HEK293 cells were incubated with 1, 10, and 25 microM arsenite [As(III)] for 6 or 24 h. Total RNA from treated and control cells was isolated, reverse transcribed, and labeled with Cy3 or Cy5, and hybridized to a human cDNA microarray. Hybridizations were performed four times using independent total RNA preparations to ensure reproducibility. Raw data from 10 and 25 microM treated cells exposed for 6 h was normalized within, and between, hybridizations followed by identification of genes affected by arsenite exposure based on practical significance (2-fold change up or down) and reproducibility (affected in four of six measurements). In these studies, 20 genes (HMOX1, MT1E, or FOSL1, etc.) were up-regulated, and 19 genes (MYC, JAK1, or CENPE, etc.) were down-regulated. Genes identified at 10 and 25 microM arsenic exposure were then examined after 1 microM treatment for 6 or 24 h. Expression of affected genes showed a dose-dependent (1-25 microM) trend that was apparently not time-dependent (6 vs. 24 h). The affected genes indicate that even this realistic, low-level arsenite exposure was recognized by the HEK293 cells (e.g. metallothionein genes) and produced an oxidative stress (e.g. heme oxygenase gene). These affected genes were characterized as stress response genes, proto-oncogene, signaling molecules, transcription factors, chemokine receptors, proteolytic enzymes, ESTs, and unknown genes. These findings imply that arsenite induces complex cellular injury and the cellular adaptation to As(III) is associated with alterations in the expression of many genes." @default.
• W2017289279 created "2016-06-24" @default.
• W2017289279 creator A5010827011 @default.
• W2017289279 creator A5028679979 @default.
• W2017289279 creator A5054621218 @default.
• W2017289279 creator A5073292702 @default.
• W2017289279 date "2003-05-01" @default.
• W2017289279 modified "2023-09-27" @default.
• W2017289279 title "Low-level arsenite induced gene expression in HEK293 cells☆" @default.
• W2017289279 cites W1494674151 @default.
• W2017289279 cites W1496912643 @default.
• W2017289279 cites W1994008801 @default.
• W2017289279 cites W2007782053 @default.
• W2017289279 cites W2013641711 @default.
• W2017289279 cites W2014679967 @default.
• W2017289279 cites W2023977270 @default.
• W2017289279 cites W2025681369 @default.
• W2017289279 cites W2038657917 @default.
• W2017289279 cites W2040100629 @default.
• W2017289279 cites W2059115578 @default.
• W2017289279 cites W2079598692 @default.
• W2017289279 cites W2084660242 @default.
• W2017289279 cites W2086201153 @default.
• W2017289279 cites W2088278787 @default.
• W2017289279 cites W2090641061 @default.
• W2017289279 cites W2090665217 @default.
• W2017289279 cites W2104699211 @default.
• W2017289279 cites W2119433742 @default.
• W2017289279 cites W2120719908 @default.
• W2017289279 cites W2129774057 @default.
• W2017289279 cites W2130562385 @default.
• W2017289279 cites W2140535485 @default.
• W2017289279 cites W2142077671 @default.
• W2017289279 cites W2154049530 @default.
• W2017289279 cites W2162114270 @default.
• W2017289279 cites W2165406168 @default.
• W2017289279 cites W2168284337 @default.
• W2017289279 cites W2170857049 @default.
• W2017289279 cites W2315716833 @default.
• W2017289279 cites W2473261551 @default.
• W2017289279 cites W1964803257 @default.
• W2017289279 doi "https://doi.org/10.1016/s0300-483x(03)00025-8" @default.
• W2017289279 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/12679051" @default.
• W2017289279 hasPublicationYear "2003" @default.
• W2017289279 type Work @default.
• W2017289279 sameAs 2017289279 @default.
• W2017289279 citedByCount "52" @default.
• W2017289279 countsByYear W20172892792013 @default.
• W2017289279 countsByYear W20172892792015 @default.
• W2017289279 countsByYear W20172892792016 @default.
• W2017289279 countsByYear W20172892792017 @default.
• W2017289279 countsByYear W20172892792018 @default.
• W2017289279 countsByYear W20172892792022 @default.
• W2017289279 crossrefType "journal-article" @default.
• W2017289279 hasAuthorship W2017289279A5010827011 @default.
• W2017289279 hasAuthorship W2017289279A5028679979 @default.
• W2017289279 hasAuthorship W2017289279A5054621218 @default.
• W2017289279 hasAuthorship W2017289279A5073292702 @default.
• W2017289279 hasConcept C104317684 @default.
• W2017289279 hasConcept C150194340 @default.
• W2017289279 hasConcept C153911025 @default.
• W2017289279 hasConcept C174510640 @default.
• W2017289279 hasConcept C178790620 @default.
• W2017289279 hasConcept C181199279 @default.
• W2017289279 hasConcept C185592680 @default.
• W2017289279 hasConcept C186836561 @default.
• W2017289279 hasConcept C187882448 @default.
• W2017289279 hasConcept C2776217839 @default.
• W2017289279 hasConcept C2776305511 @default.
• W2017289279 hasConcept C2779219270 @default.
• W2017289279 hasConcept C2779730776 @default.
• W2017289279 hasConcept C2780091579 @default.
• W2017289279 hasConcept C502230775 @default.
• W2017289279 hasConcept C54355233 @default.
• W2017289279 hasConcept C55493867 @default.
• W2017289279 hasConcept C67705224 @default.
• W2017289279 hasConcept C81885089 @default.
• W2017289279 hasConcept C86803240 @default.
• W2017289279 hasConceptScore W2017289279C104317684 @default.
• W2017289279 hasConceptScore W2017289279C150194340 @default.
• W2017289279 hasConceptScore W2017289279C153911025 @default.
• W2017289279 hasConceptScore W2017289279C174510640 @default.
• W2017289279 hasConceptScore W2017289279C178790620 @default.
• W2017289279 hasConceptScore W2017289279C181199279 @default.
• W2017289279 hasConceptScore W2017289279C185592680 @default.
• W2017289279 hasConceptScore W2017289279C186836561 @default.
• W2017289279 hasConceptScore W2017289279C187882448 @default.
• W2017289279 hasConceptScore W2017289279C2776217839 @default.
• W2017289279 hasConceptScore W2017289279C2776305511 @default.
• W2017289279 hasConceptScore W2017289279C2779219270 @default.
• W2017289279 hasConceptScore W2017289279C2779730776 @default.
• W2017289279 hasConceptScore W2017289279C2780091579 @default.
• W2017289279 hasConceptScore W2017289279C502230775 @default.
• W2017289279 hasConceptScore W2017289279C54355233 @default.
• W2017289279 hasConceptScore W2017289279C55493867 @default.
• W2017289279 hasConceptScore W2017289279C67705224 @default.
• W2017289279 hasConceptScore W2017289279C81885089 @default.
• W2017289279 hasConceptScore W2017289279C86803240 @default.

• next