FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2017292039> ?p ?o ?g. }

• W2017292039 endingPage "14278" @default.
• W2017292039 startingPage "14267" @default.
• W2017292039 abstract "The binding of N-cyclohexylformamide (CXF) to the complex of horse liver alcohol dehydrogenase with NADH mimics that of the Michaelis complex for aldehyde reduction catalyzed by the enzyme. The Raman spectra of bound CXF and its 13C- and 15N-substituted derivatives have been obtained using Raman difference techniques, and the results are compared with CXF spectra in aqueous solution and in methylene chloride. The results indicate that the amide N-H bond is trans to the C=O bond of CXF both in solution and in the enzyme ternary complex. The C=O stretch and N-H bending modes of the amide of CXF shift -16 and -9 cm-1, respectively, in the enzyme ternary complex relative to that in aqueous solution and -48 and 36 cm-1, respectively, relative to that in methylene chloride. Ab initio normal mode calculations on various model systems of CXF show that the observed frequency changes of the C=O stretch mode have contributions from the frequency changes induced by the environmental changes near both the local C=O bond and the remote N-H bond. The same is true for the observed N-H bending frequency change. Our calculations also show that the environmentally induced frequency changes are additive so that it is possible to determine the C=O stretch (or N-H bending) frequency change that is due to the local interaction change near the C=O (or N-H) bond from the observed frequency changes. On the basis of these results and the empirical relationship between the C=O stretch frequency shift and the interaction enthalpy change on the C=O bond developed here, it is found that the C=O group of CXF in the enzyme/NADH/CXF complex binds with a favorable interaction enthalpy of approximately 5.5 kcal/mol relative to water. Similar analysis suggests that the N-H moiety of CXF is destabilized in the ternary complex by about 1.5 kcal/mol relative to water but is stabilized by about 1.5 kcal/mol relative to a hydrophobic environment. The analysis describes quantitatively the binding of the C=O of CXF with the catalytic zinc and the hydroxyl group of Ser-48 and the interaction of the N-H with the benzene ring of Phe-93 of the enzyme." @default.
• W2017292039 created "2016-06-24" @default.
• W2017292039 creator A5000885435 @default.
• W2017292039 creator A5013681613 @default.
• W2017292039 creator A5015206461 @default.
• W2017292039 creator A5065719969 @default.
• W2017292039 creator A5073476505 @default.
• W2017292039 date "1998-09-18" @default.
• W2017292039 modified "2023-10-16" @default.
• W2017292039 title "A Raman Spectroscopic Characterization of Bonding in the Complex of Horse Liver Alcohol Dehydrogenase with NADH and <i>N</i>-Cyclohexylformamide" @default.
• W2017292039 cites W1986216396 @default.
• W2017292039 cites W2000309760 @default.
• W2017292039 cites W2008832827 @default.
• W2017292039 cites W2014698996 @default.
• W2017292039 cites W2018116455 @default.
• W2017292039 cites W2031885198 @default.
• W2017292039 cites W2037254453 @default.
• W2017292039 cites W2170040939 @default.
• W2017292039 doi "https://doi.org/10.1021/bi981477e" @default.
• W2017292039 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/9760265" @default.
• W2017292039 hasPublicationYear "1998" @default.
• W2017292039 type Work @default.
• W2017292039 sameAs 2017292039 @default.
• W2017292039 citedByCount "27" @default.
• W2017292039 countsByYear W20172920392012 @default.
• W2017292039 countsByYear W20172920392013 @default.
• W2017292039 countsByYear W20172920392014 @default.
• W2017292039 countsByYear W20172920392015 @default.
• W2017292039 countsByYear W20172920392017 @default.
• W2017292039 countsByYear W20172920392019 @default.
• W2017292039 countsByYear W20172920392020 @default.
• W2017292039 countsByYear W20172920392021 @default.
• W2017292039 countsByYear W20172920392022 @default.
• W2017292039 countsByYear W20172920392023 @default.
• W2017292039 crossrefType "journal-article" @default.
• W2017292039 hasAuthorship W2017292039A5000885435 @default.
• W2017292039 hasAuthorship W2017292039A5013681613 @default.
• W2017292039 hasAuthorship W2017292039A5015206461 @default.
• W2017292039 hasAuthorship W2017292039A5065719969 @default.
• W2017292039 hasAuthorship W2017292039A5073476505 @default.
• W2017292039 hasConcept C120665830 @default.
• W2017292039 hasConcept C121332964 @default.
• W2017292039 hasConcept C147789679 @default.
• W2017292039 hasConcept C155647269 @default.
• W2017292039 hasConcept C178790620 @default.
• W2017292039 hasConcept C181199279 @default.
• W2017292039 hasConcept C184651966 @default.
• W2017292039 hasConcept C185592680 @default.
• W2017292039 hasConcept C199360897 @default.
• W2017292039 hasConcept C2777344606 @default.
• W2017292039 hasConcept C2777454769 @default.
• W2017292039 hasConcept C2778439535 @default.
• W2017292039 hasConcept C2781066024 @default.
• W2017292039 hasConcept C2781442258 @default.
• W2017292039 hasConcept C31684184 @default.
• W2017292039 hasConcept C40003534 @default.
• W2017292039 hasConcept C41008148 @default.
• W2017292039 hasConcept C64452783 @default.
• W2017292039 hasConcept C71240020 @default.
• W2017292039 hasConcept C8010536 @default.
• W2017292039 hasConceptScore W2017292039C120665830 @default.
• W2017292039 hasConceptScore W2017292039C121332964 @default.
• W2017292039 hasConceptScore W2017292039C147789679 @default.
• W2017292039 hasConceptScore W2017292039C155647269 @default.
• W2017292039 hasConceptScore W2017292039C178790620 @default.
• W2017292039 hasConceptScore W2017292039C181199279 @default.
• W2017292039 hasConceptScore W2017292039C184651966 @default.
• W2017292039 hasConceptScore W2017292039C185592680 @default.
• W2017292039 hasConceptScore W2017292039C199360897 @default.
• W2017292039 hasConceptScore W2017292039C2777344606 @default.
• W2017292039 hasConceptScore W2017292039C2777454769 @default.
• W2017292039 hasConceptScore W2017292039C2778439535 @default.
• W2017292039 hasConceptScore W2017292039C2781066024 @default.
• W2017292039 hasConceptScore W2017292039C2781442258 @default.
• W2017292039 hasConceptScore W2017292039C31684184 @default.
• W2017292039 hasConceptScore W2017292039C40003534 @default.
• W2017292039 hasConceptScore W2017292039C41008148 @default.
• W2017292039 hasConceptScore W2017292039C64452783 @default.
• W2017292039 hasConceptScore W2017292039C71240020 @default.
• W2017292039 hasConceptScore W2017292039C8010536 @default.
• W2017292039 hasIssue "40" @default.
• W2017292039 hasLocation W20172920391 @default.
• W2017292039 hasLocation W20172920392 @default.
• W2017292039 hasOpenAccess W2017292039 @default.
• W2017292039 hasPrimaryLocation W20172920391 @default.
• W2017292039 hasRelatedWork W1967309651 @default.
• W2017292039 hasRelatedWork W1968310998 @default.
• W2017292039 hasRelatedWork W1979627953 @default.
• W2017292039 hasRelatedWork W1984580649 @default.
• W2017292039 hasRelatedWork W1985368379 @default.
• W2017292039 hasRelatedWork W1990735481 @default.
• W2017292039 hasRelatedWork W1994847533 @default.
• W2017292039 hasRelatedWork W2092845367 @default.
• W2017292039 hasRelatedWork W2316617565 @default.
• W2017292039 hasRelatedWork W2950176770 @default.
• W2017292039 hasVolume "37" @default.
• W2017292039 isParatext "false" @default.
• W2017292039 isRetracted "false" @default.

• next